PRPF19 mRNA 编码一个小的开放阅读框，对人类细胞的存活很重要。
PRPF19 mRNA Encodes a Small Open Reading Frame That Is Important for Viability of Human Cells.
机构信息
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
Department of Chemistry, Moscow State University, Moscow, Russia.
出版信息
Dokl Biochem Biophys. 2024 Apr;515(1):41-47. doi: 10.1134/S1607672923700722. Epub 2024 Mar 12.
High-throughput ribosome profiling demonstrates the translation of thousands of small open reading frames located in the 5' untranslated regions of messenger RNAs (upstream ORFs). Upstream ORF can both perform a regulatory function by influencing the translation of the downstream main ORF and encode a small functional protein or microprotein. In this work, we showed that the 5' untranslated region of the PRPF19 mRNA encodes an upstream ORF that is translated in human cells. Inactivation of this upstream ORF reduces the viability of human cells.
高通量核糖体分析表明，数千个位于信使 RNA（上游开放阅读框）5'非翻译区的小开放阅读框可被翻译。上游开放阅读框可通过影响下游主开放阅读框的翻译来发挥调节功能，也可编码小功能蛋白或微蛋白。在这项工作中，我们表明 PRPF19 mRNA 的 5'非翻译区编码一个可在人类细胞中翻译的上游开放阅读框。该上游开放阅读框失活会降低人类细胞的活力。
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