Department of Biology, College of Sciences, Shiraz University, Shiraz, 71467-13565, Iran.
Pathol Oncol Res. 2020 Jan;26(1):335-339. doi: 10.1007/s12253-018-0470-0. Epub 2018 Sep 21.
To determine the association between the SOD1 (Ins/Del), SOD2 (rs2758339, rs5746136), and SOD3 (rs2536512) polymorphisms and the risk of gastric cancer the present study performed. This is a case-control study, including 159 patients with gastric cancer and 242 healthy controls. All subjects were Persian Muslims living in Shiraz (south west Iran). Frequency matching by age and gender was performed. Genomic DNA was extracted from whole blood. Genotypes of the study polymorphism were determined using polymerase chain reaction based methods. The SOD1 Ins/Del and SOD3 rs2536512 polymorphisms did not appear to have relationship with gastric cancer risk. Both SOD2 polymorphisms (rs2758339, rs5746136) showed significant association with the risk of gastric cancer, under assumption that the variant alleles act as dominant alleles. There was significant association between smoking habit and the risk of gastric cancer (OR = 2.54, 95% CI = 1.61-4.02, P < 0.001). Smoker individuals having two putative high-risk genotypes showed elevated risk of gastric cancer compared with nonsmokers without high-risk genotypes, (OR = 5.75, 95% CI = 1.59-20.6, P = 0.007). Assuming that smoking habit and the genotypes are independent risk factors, there was a significant linear trend for the numbers of risk factors and gastric cancer risk (χ = 22.9, P < 0.001). This study indicates that the SOD2 polymorphism (rs2758339, rs5746136) is associated with increased risk of gastric cancer, especially in smoker individuals.
为了确定 SOD1（Ins/Del）、SOD2（rs2758339、rs5746136）和 SOD3（rs2536512）多态性与胃癌风险之间的关联，本研究进行了。这是一项病例对照研究，包括 159 例胃癌患者和 242 例健康对照。所有受试者均为居住在设拉子（伊朗西南部）的波斯穆斯林。通过年龄和性别进行频率匹配。从全血中提取基因组 DNA。使用聚合酶链反应（PCR）方法确定研究多态性的基因型。SOD1 Ins/Del 和 SOD3 rs2536512 多态性似乎与胃癌风险无关。SOD2 两个多态性（rs2758339、rs5746136）均表现出与胃癌风险的显著关联，假设变异等位基因作为显性等位基因。吸烟习惯与胃癌风险之间存在显著关联（OR=2.54，95%CI=1.61-4.02，P<0.001）。与不吸烟者无高危基因型相比，具有两种假定高危基因型的吸烟者个体患胃癌的风险增加（OR=5.75，95%CI=1.59-20.6，P=0.007）。假设吸烟习惯和基因型是独立的危险因素，危险因素的数量与胃癌风险之间存在显著的线性趋势（χ²=22.9，P<0.001）。本研究表明，SOD2 多态性（rs2758339、rs5746136）与胃癌风险增加相关，尤其是在吸烟者中。
