Department of Internal Medicine, The First Teaching Hospital of Jilin University, Changchun 130021, Jilin Province, China.
World J Gastroenterol. 2012 Sep 7;18(33):4522-32. doi: 10.3748/wjg.v18.i33.4522.
To investigate the genomic copy number alterations that may harbor key driver genes in gastric tumorigenesis.
Using high-resolution array comparative genomic hybridization (CGH), we investigated the genomic alterations of 20 advanced primary gastric adenocarcinomas (seventeen tubular and three mucinous) of Chinese patients from the Jilin province. Ten matching adjacent normal regions from the same patients were also studied.
The most frequent imbalances detected in these cancer samples were gains of 3q26.31-q27.2, 5p, 8q, 11p, 18p, 19q and 20q and losses of 3p, 4p, 18q and 21q. The use of high-resolution array CGH increased the resolution and sensitivity of the observed genomic changes and identified focal genetic imbalances, which included 54 gains and 16 losses that were smaller than 1 Mb in size. The most interesting focal imbalances were the intergenic loss/homozygous deletion of the fragile histidine triad gene and the amplicons 11q13, 18q11.2 and 19q12, as well as the novel amplicons 1p36.22 and 11p15.5.
These regions, especially the focal amplicons, may harbor key driver genes that will serve as biomarkers for either the diagnosis or the prognosis of gastric cancer, and therefore, a large-scale investigation is recommended.
研究可能在胃癌发生中具有关键驱动基因的基因组拷贝数改变。
使用高分辨率阵列比较基因组杂交（CGH），我们研究了来自吉林省的 20 例晚期原发性胃腺癌（17 例管状和 3 例黏液性）的中国患者的基因组改变。还研究了来自同一患者的 10 个匹配的相邻正常区域。
在这些癌症样本中检测到的最常见的不平衡是 3q26.31-q27.2、5p、8q、11p、18p、19q 和 20q 的增益，以及 3p、4p、18q 和 21q 的缺失。高分辨率阵列 CGH 的使用提高了观察到的基因组变化的分辨率和灵敏度，并确定了焦点遗传不平衡，其中包括 54 个增益和 16 个小于 1 Mb 的缺失。最有趣的焦点不平衡是脆性组氨酸三联体基因的基因间缺失/纯合缺失，以及 11q13、18q11.2 和 19q12 的扩增子，以及新的 1p36.22 和 11p15.5 的扩增子。
这些区域，特别是焦点扩增子，可能含有关键的驱动基因，可作为胃癌诊断或预后的生物标志物，因此建议进行大规模的研究。
