Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, 600 036, India.
Department of Orthopedics, Centre for Stem Cell Research, Christian Medical College, Vellore, 632004, India.
Colloids Surf B Biointerfaces. 2018 Dec 1;172:690-698. doi: 10.1016/j.colsurfb.2018.09.010. Epub 2018 Sep 6.
This current study is aimed towards the fabrication of AZ31 magnesium cylindrical mesh cage implant with circular holes for orthopedic applications. This mesh cage is coated with nanocomposite material containing polycaprolactone (PCL), pluronic F127 and nano hydroxyapatite (nHA) by electrospinning process. Morphology and composition were analyzed by various characterization techniques. Controlled degradation and weight loss of the nanocomposite coated samples in 28 days were observed when compared with uncoated samples in SBF (simulated body fluid). The nanocomposite coated material was not cytotoxic to MG63 osteosarcoma cells. The cell viability, morphology, ALP activity, calcium mineralization and collagen deposition were also better on this when compared to uncoated. Smooth and randomly deposited nanofibers on the mesh cage was observed and the contact angle indicated that the surface is hydrophilic with (initial contact angle of 55 ± 1° and after 10 s 0°) when compared to PCL (99°) coated surface. 2-5 fold higher mRNA expression levels of osteogenic genes namely ALP, BMP2, COL1 and RUNX2 was observed with nanocomposite coated scaffolds than uncoated and PCL coated samples in 14 days. These results indicate the potential use of the nanocomposite coated AZ31 cylindrical mesh cage for segmental bone defect repair and can be used as a degradable implant for orthopedic applications.
本研究旨在制备用于骨科应用的具有圆形孔的 AZ31 镁圆柱状网笼植入物。该网笼通过静电纺丝工艺涂覆了包含聚己内酯 (PCL)、泊洛沙姆 F127 和纳米羟基磷灰石 (nHA) 的纳米复合材料。通过各种表征技术分析了形态和组成。与在 SBF（模拟体液）中的未涂层样品相比，在 28 天内观察到纳米复合材料涂层样品的受控降解和重量损失。纳米复合材料涂层材料对 MG63 骨肉瘤细胞无细胞毒性。与未涂层相比，细胞活力、形态、碱性磷酸酶活性、钙矿化和胶原蛋白沉积也更好。观察到网笼上的纳米纤维光滑且随机沉积，接触角表明表面具有亲水性（初始接触角为 55±1°，10 秒后为 0°），而 PCL（99°）涂层表面的接触角为 99°。与未涂层和 PCL 涂层样品相比，在 14 天内，纳米复合材料涂层支架的成骨基因（ALP、BMP2、COL1 和 RUNX2）的 mRNA 表达水平高 2-5 倍。这些结果表明，纳米复合材料涂层的 AZ31 圆柱状网笼具有用于节段性骨缺损修复的潜力，并可用作骨科应用的可降解植入物。
