Department of Oral Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, Study Group: Biomaterials/Surfaces, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
BerlinAnalytix GmbH, 12109 Berlin, Germany.
Int J Mol Sci. 2020 Apr 28;21(9):3098. doi: 10.3390/ijms21093098.
Bioresorbable collagenous barrier membranes are used to prevent premature soft tissue ingrowth and to allow bone regeneration. For volume stable indications, only non-absorbable synthetic materials are available. This study investigates a new bioresorbable hydrofluoric acid (HF)-treated magnesium (Mg) mesh in a native collagen membrane for volume stable situations.
HF-treated and untreated Mg were compared in direct and indirect cytocompatibility assays. In vivo, 18 New Zealand White Rabbits received each four 8 mm calvarial defects and were divided into four groups: (a) HF-treated Mg mesh/collagen membrane, (b) untreated Mg mesh/collagen membrane (c) collagen membrane and (d) sham operation. After 6, 12 and 18 weeks, Mg degradation and bone regeneration was measured using radiological and histological methods.
In vitro, HF-treated Mg showed higher cytocompatibility. Histopathologically, HF-Mg prevented gas cavities and was degraded by mononuclear cells via phagocytosis up to 12 weeks. Untreated Mg showed partially significant more gas cavities and a fibrous tissue reaction. Bone regeneration was not significantly different between all groups.
HF-Mg meshes embedded in native collagen membranes represent a volume stable and biocompatible alternative to the non-absorbable synthetic materials. HF-Mg shows less corrosion and is degraded by phagocytosis. However, the application of membranes did not result in higher bone regeneration.
生物可吸收胶原屏障膜用于防止软组织过早向内生长，并允许骨再生。对于体积稳定的适应症，仅可使用不可吸收的合成材料。本研究调查了一种新的生物可吸收氢氟酸（HF）处理的镁（Mg）网在天然胶原膜中的应用，用于体积稳定的情况。
在直接和间接细胞相容性测定中比较了 HF 处理和未处理的 Mg。在体内，18 只新西兰白兔每只接受 4 个 8mm 颅骨缺损，并分为 4 组：（a）HF 处理的 Mg 网/胶原膜，（b）未处理的 Mg 网/胶原膜，（c）胶原膜和（d）假手术。在 6、12 和 18 周后，使用放射学和组织学方法测量 Mg 的降解和骨再生。
体外，HF 处理的 Mg 显示出更高的细胞相容性。组织病理学上，HF-Mg 防止了气腔的形成，并在 12 周内被单核细胞通过吞噬作用降解。未处理的 Mg 显示出部分气腔和纤维组织反应更明显。所有组之间的骨再生没有显著差异。
嵌入天然胶原膜中的 HF-Mg 网是一种可替代不可吸收合成材料的体积稳定和生物相容性材料。HF-Mg 显示出较少的腐蚀，并通过吞噬作用降解。然而，膜的应用并没有导致更高的骨再生。
