Lokesh B R, Cunningham M L
Toxicol Lett. 1986 Nov;34(1):75-84. doi: 10.1016/0378-4274(86)90147-5.
Potassium superoxide (KO2) forms superoxide anion (O2-) in aqueous medium as measured by the superoxide dismutase (SOD)-inhibitable cytochrome c reduction assay of McCord and Fridovich. The reduction of cytochrome c by O2- formed by KO2 was observed only above pH 7.0 and demonstrated a pH optimum at pH 9.6. SOD was an effective inhibitor of the cytochrome c reduction produced by KO2. Hydroxyl radical scavengers and singlet oxygen quenchers did not interfere with the reduction of cytochrome c by KO2. These data demonstrate that addition of KO2 to aqueous medium is an easy chemical method for the production of O2- in controlled amounts.
通过麦考德和弗里多维奇的超氧化物歧化酶（SOD）抑制的细胞色素c还原测定法测量，超氧化钾（KO₂）在水性介质中形成超氧阴离子（O₂⁻）。仅在pH 7.0以上观察到由KO₂形成的O₂⁻对细胞色素c的还原作用，并且在pH 9.6时表现出最佳pH值。SOD是KO₂产生的细胞色素c还原的有效抑制剂。羟基自由基清除剂和单线态氧猝灭剂不干扰KO₂对细胞色素c的还原。这些数据表明，向水性介质中添加KO₂是一种简单的化学方法，可用于以可控量产生O₂⁻。
