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通过线粒体DNA的限制性内切酶切割模式检测污染的细胞系

Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA.

作者信息

Grossman L I, Parker R C, Watson R M, Chandler S E, Teplitz M

出版信息

Nucleic Acids Res. 1977;4(5):1267-71. doi: 10.1093/nar/4.5.1267.

DOI:10.1093/nar/4.5.1267
PMID:302438
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343754/
Abstract

A putative HeLa cell culture line was discovered to be contaminated with mouse cells by examination of agarose gel profiles of restriction endonuclease digests of mitochondrial DNA. The contamination was confirmed by karyotypic analysis, and by observation of the mouse satellite band in an analytical buoyant density centrifugation of total cellular DNA. Restriction endonuclease analysis of mitochondrial DNA is suggested as a useful method for monitoring the species of cells in culture.

摘要

通过对线粒体DNA限制性内切酶消化产物的琼脂糖凝胶图谱进行检查，发现一个假定的海拉细胞系被小鼠细胞污染。通过核型分析以及在总细胞DNA的分析性浮力密度离心实验中观察到小鼠卫星带，证实了这种污染。线粒体DNA的限制性内切酶分析被认为是监测培养细胞种类的一种有用方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0b4/343754/437b16aead93/nar00478-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0b4/343754/437b16aead93/nar00478-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0b4/343754/437b16aead93/nar00478-0117-a.jpg

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Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA.通过线粒体DNA的限制性内切酶切割模式检测污染的细胞系
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2
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引用本文的文献

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HeLa cell identification by analysis of ribosomal DNA segment patterns generated by endonuclease restriction.
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本文引用的文献

1
Homology and structural relationships between the dimeric and monomeric circular forms of mitochondrial DNA from human leukemic leukocytes.来自人类白血病白细胞的线粒体DNA二聚体和单体环状形式之间的同源性及结构关系。
J Mol Biol. 1970 Jan 28;47(2):137-53. doi: 10.1016/0022-2836(70)90335-9.
2
Genetic markers as tracers in cell culture.细胞培养中作为示踪剂的遗传标记物。
Natl Cancer Inst Monogr. 1967 Sep;26:167-95.
3
The number of mitochondrial deoxyribonucleic acid genomes in mouse L and human HeLa cells. Quantitative isolation of mitochondrial deoxyribonucleic acid.
小鼠L细胞和人宫颈癌细胞系HeLa细胞中线粒体脱氧核糖核酸基因组的数量。线粒体脱氧核糖核酸的定量分离。
J Biol Chem. 1974 Dec 25;249(24):7991-5.
4
Banded marker chromosomes as indicators of intraspecies cellular contamination.带型标记染色体作为种内细胞污染的指标。
Science. 1974 Jun 7;184(4141):1093-6. doi: 10.1126/science.184.4141.1093.
5
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.用EcoRI限制性内切酶切割噬菌体λ DNA的研究。
J Mol Biol. 1975 Jan 25;91(3):315-28. doi: 10.1016/0022-2836(75)90383-6.
6
Specific cleavage analysis of mammalian mitochondrial DNA.哺乳动物线粒体DNA的特异性切割分析
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4496-500. doi: 10.1073/pnas.72.11.4496.
7
Restriction endonuclease cleavage maps of rat and mouse mitochondrial DNAs.大鼠和小鼠线粒体DNA的限制性内切酶切割图谱。
Nucleic Acids Res. 1977;4(5):1291-9. doi: 10.1093/nar/4.5.1291.
8
A restriction endonuclease cleavage map of mouse mitochondrial DNA.小鼠线粒体DNA的限制性内切核酸酶切割图谱。
Nucleic Acids Res. 1977;4(5):1273-89. doi: 10.1093/nar/4.5.1273.