通过线粒体DNA的限制性内切酶切割模式检测污染的细胞系
Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA.
作者信息
Grossman L I, Parker R C, Watson R M, Chandler S E, Teplitz M
出版信息
Nucleic Acids Res. 1977;4(5):1267-71. doi: 10.1093/nar/4.5.1267.
Abstract
A putative HeLa cell culture line was discovered to be contaminated with mouse cells by examination of agarose gel profiles of restriction endonuclease digests of mitochondrial DNA. The contamination was confirmed by karyotypic analysis, and by observation of the mouse satellite band in an analytical buoyant density centrifugation of total cellular DNA. Restriction endonuclease analysis of mitochondrial DNA is suggested as a useful method for monitoring the species of cells in culture.
摘要
通过对线粒体DNA限制性内切酶消化产物的琼脂糖凝胶图谱进行检查，发现一个假定的海拉细胞系被小鼠细胞污染。通过核型分析以及在总细胞DNA的分析性浮力密度离心实验中观察到小鼠卫星带，证实了这种污染。线粒体DNA的限制性内切酶分析被认为是监测培养细胞种类的一种有用方法。
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