Moore K H, Johnson P H, Chandler S E, Grossman L I
Nucleic Acids Res. 1977;4(5):1273-89. doi: 10.1093/nar/4.5.1273.
A restriction endonuclease cleavage map is presented for mouse mitochondrial DNA. This map was constructed by electron microscopic measurements on partial digests containing fixed D-loops, and by electrophoretic analysis of partial and complete single enzyme digests, and of double digests. No map differences were detected between mitochondrial DNA from cultured LA9 cells and an inbred mouse line for the six endonucleases used. Three cleavage sites recognized by HpaI, five sites recognized by HincII, two sites recognized PstI and four sites recognized by BamI were located with respect to the origin of replication and the EcoRI and HinIII sites previously determined by others. No cleavages were produced by KpnI or SalI. The migration of linear DNA with a molecular weight greater than 1 X 10(6) was not a linear function of log molecular weight in 1% agarose gels run at 6.6 volts/cm.
本文给出了小鼠线粒体DNA的限制性内切酶切割图谱。该图谱是通过对含有固定D环的部分消化产物进行电子显微镜测量，以及对部分和完全单酶消化产物及双酶消化产物进行电泳分析构建而成的。在所使用的六种内切酶方面，未检测到来自培养的LA9细胞的线粒体DNA与近交系小鼠之间的图谱差异。相对于复制起点以及先前其他人确定的EcoRI和HinIII位点，定位了三个被HpaI识别的切割位点、五个被HincII识别的位点、两个被PstI识别的位点以及四个被BamI识别的位点。KpnI或SalI未产生切割。在6.6伏/厘米的条件下于1%琼脂糖凝胶中运行时，分子量大于1×10(6)的线性DNA的迁移不是对数分子量的线性函数。
