Ejaz Muhammad, Raja Naveed Iqbal, Mashwani Zia-Ur-Rehman, Ahmad Muhammad Sheeraz, Hussain Mubashir, Iqbal Muhammad
Department of Botany, PMAS Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan.
Department of Biochemistry, PMAS Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan.
IET Nanobiotechnol. 2018 Oct;12(7):927-932. doi: 10.1049/iet-nbt.2018.0057.
This study was organised to check the effect of silver nanoparticles and silver nitrate on rice growth against biotic stress. Silver nanoparticles were synthesised by using plant extract as reducing agent, followed by characterisation through UV Vis spectroscopy, XRD, EDS and SEM. Aspergillus application significantly reduced rice plant fresh mass (0.9%), dry mass (0.21%), root length (2.3%), shoot length (5.2%) and root number (1%) in comparison to control. Similarly, leaf area, leaf fresh mass, dry mass and leaf number were also reduced by 23.1, 0.02, 0.11 and 0.9%, respectively. AgNPs and AgNO treatments increased the root length (16.2 & 12.8%), shoot length (21 & 20%), root number (8.1 & 6.8%), plant fresh weight (6.4 & 5%) and plant dry weight (4.6 & 3.5%) in 75mg/l treatment of AgNPs and AgNO respectively. Similarly, AgNPs and AgNO treatment (75 mg/l concentrations) reflected remarkable increase in leaf area (58.8 & 57.2 %), leaf number (4.3 & 3.7 %), leaf fresh weight (1.7 & 1.4 %) and leaf dry weight (0.9 & 0.8 %). Overall AgNPs showed more significant results as compared to AgNO. The quantity of aflatoxins ranged from 3.1 to 7.7 μg/kg against tolerable limit (4 µg/kg). Overall AgNPs and AgNO treatments showed significant results and it could be considered as a strategy for aflatoxin management in rice plants.
本研究旨在检测银纳米颗粒和硝酸银对水稻生长抗生物胁迫的影响。采用植物提取物作为还原剂合成银纳米颗粒,随后通过紫外可见光谱、X射线衍射、能谱和扫描电子显微镜进行表征。与对照相比,接种曲霉菌显著降低了水稻植株的鲜重(0.9%)、干重(0.21%)、根长(2.3%)、地上部长度(5.2%)和根数(1%)。同样,叶面积、叶鲜重、叶干重和叶片数也分别减少了23.1%、0.02%、0.11%和0.9%。在75mg/l的银纳米颗粒和硝酸银处理中,银纳米颗粒和硝酸银处理分别使根长增加了16.2%和12.8%,地上部长度增加了21%和20%,根数增加了8.1%和6.8%,植株鲜重增加了6.4%和5%,植株干重增加了4.6%和3.5%。同样,银纳米颗粒和硝酸银处理(75mg/l浓度)使叶面积显著增加(58.8%和57.2%)、叶片数显著增加(4.3%和3.7%)、叶鲜重显著增加(1.7%和1.4%)、叶干重显著增加(0.9%和0.8%)。总体而言,与硝酸银相比,银纳米颗粒的效果更显著。黄曲霉毒素的含量在3.1至7.7μg/kg之间,超过了可耐受限量(4μg/kg)。总体而言,银纳米颗粒和硝酸银处理均显示出显著效果,可被视为水稻植株黄曲霉毒素管理的一种策略。