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使用多种植物提取物的银纳米颗粒的植物合成、表征及体外抗菌活性

Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts.

作者信息

Ahmad Bilal, Chang Li, Satti Usama Qamar, Rehman Sami Ur, Arshad Huma, Mustafa Ghazala, Shaukat Uzma, Wang Fenghua, Tong Chunyi

机构信息

College of Biology, Hunan University, Changsha 410082, China.

College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.

出版信息

Bioengineering (Basel). 2022 Dec 7;9(12):779. doi: 10.3390/bioengineering9120779.

Abstract

, (mint), (coriander), and (lemongrass) leaf extracts were used to synthesize stable silver nanoparticles (Ag-NPs) by green chemistry. UV-vis spectrophotometry, X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques were used to characterize these biosynthesized nanoparticles. The data indicated that the silver nanoparticles were successfully synthesized, and the narrower particle size distribution was at 10-22 nm by maintaining a specific pH. As a short-term post-sowing treatment, Ag-NP solutions of different sizes (10 and 50 ppm) were introduced to mung bean seedlings, and the overall increase in plant growth was found to be more pronounced at 50 ppm concentration. The antibacterial activity of Ag-NPs was also investigated by disc diffusion test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. The zones of inhibition (ZOI) were shown by () (1.9, 2.1, 1.7, and 2 mm), followed by () (1.8, 1.7, 1.6, and 1.9 mm), against coriander, mint, , and lemongrass, respectively. MIC and MBC values of and ranged from 7 to 8 µg/mL. Overall, this study demonstrates that Ag-NPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agent for the treatment of bacterial infection.

摘要

薄荷、香菜和柠檬草叶提取物通过绿色化学用于合成稳定的银纳米颗粒(Ag-NPs)。采用紫外可见分光光度法、X射线衍射(XRD)、热重分析(TGA)、扫描电子显微镜(SEM)和能量色散X射线(EDX)光谱技术对这些生物合成的纳米颗粒进行表征。数据表明,通过维持特定pH值成功合成了银纳米颗粒,且粒径分布较窄,为10 - 22 nm。作为播种后的短期处理,将不同尺寸(10和50 ppm)的Ag-NP溶液施用于绿豆幼苗,发现浓度为50 ppm时植物生长的总体增加更为明显。还通过纸片扩散试验、最低抑菌浓度(MIC)和最低杀菌浓度(MBC)试验研究了Ag-NPs的抗菌活性。分别对香菜、薄荷、柠檬草和柠檬草显示出抑菌圈(ZOI),依次为(1.9、2.1、1.7和2 mm)、(1.8、1.7、1.6和1.9 mm)。和的MIC和MBC值范围为7至8 µg/mL。总体而言,本研究表明Ag-NPs具有很强的抗菌活性,因此可能被开发为一种新型抗菌剂用于治疗细菌感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bffb/9774305/ae6817fee6e8/bioengineering-09-00779-sch001.jpg

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