Nathan P D, Walker L, Hardie D, Richardson P, Khan M, Johnson G D, Ling N R
Clin Exp Immunol. 1986 Jul;65(1):112-9.
A mouse monoclonal antibody named BU11 which detects an antigen strongly expressed on human plasma cells is described. The antibody stains plasma cells in tonsil sections, fresh and cultured plasmacytoid cells from the bone marrow of patients with multiple myeloma and cells of the plasmacytoid cell line RPMI 8226 used as the immunogen. In vitro studies of pokeweed mitogen (PWM) stimulated peripheral blood B cells and Epstein-Barr virus (EBV) stimulated tonsil B cells show that the antigen is present mainly on cells coexpressing the OKT10 antigen and containing cytoplasmic immunoglobulin (cIg). The BU11 antigen is expressed weakly on some normal B cells and is not present on T cells, monocytes or granulocytes. The antigen is of molecular weight 58kD under reducing conditions and is biochemically distinct from previously described plasma cell antigens.
本文描述了一种名为BU11的小鼠单克隆抗体，它能检测到人浆细胞上强烈表达的一种抗原。该抗体可对扁桃体切片中的浆细胞、多发性骨髓瘤患者骨髓中的新鲜和培养的浆细胞样细胞以及用作免疫原的浆细胞样细胞系RPMI 8226的细胞进行染色。对商陆有丝分裂原（PWM）刺激的外周血B细胞和爱泼斯坦-巴尔病毒（EBV）刺激的扁桃体B细胞的体外研究表明，该抗原主要存在于共表达OKT10抗原并含有细胞质免疫球蛋白（cIg）的细胞上。BU11抗原在一些正常B细胞上弱表达，在T细胞、单核细胞或粒细胞上不存在。在还原条件下，该抗原的分子量为58kD，在生化性质上与先前描述的浆细胞抗原不同。
