枸杞花粉中主要多糖的分离、鉴定及其对 DU145 细胞的抗肿瘤作用。
Isolation, Characterization and Antitumor Effect on DU145 Cells of a Main Polysaccharide in Pollen of Chinese Wolfberry.
机构信息
College of Public Health and Management, Ningxia Medical University, Yinchuan 750004, China.
Laboratory Animal Center, Ningxia Medical University, Yinchuan 750004, China.
出版信息
Molecules. 2018 Sep 21;23(10):2430. doi: 10.3390/molecules23102430.
Modern studies have shown that pollen has a certain role in the treatment of prostate-related diseases. In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, further purified by chromatography on a DEAE-cellulose column and Sephadex G-100 column. Homogeneous polysaccharide CF1 of WPPS was obtained, the molecular weight of which was estimated to be 1540.10 ± 48.78 kDa by HPGPC-ELSD. HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of CF1 were mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose, in a molar ratio of 0.68:0.59:0.27:0.24:0.22:0.67:0.08. The antitumor effects of CF1 upon MTT, Tunel assay and flow cytometry assay were investigated in vitro. The results showed that CF1 exhibited a dose-dependent antiproliferative effect, with an IC50 value of 374.11 μg/mL against DU145 prostate cancer cells. Tunel assay and flow cytometry assay showed that the antitumor activity of CF1 was related to apoptosis in vitro. The present study suggested that the CF1 of WPPs might be a potential source of antitumor functional food or agent.
现代研究表明花粉在治疗前列腺相关疾病方面具有一定作用。本研究采用热水浸提法提取枸杞花粉多糖（WPPs），经 DEAE-纤维素柱和 Sephadex G-100 柱层析进一步纯化，得到均一多糖 CF1，经高效凝胶渗透色谱-蒸发光散射检测（HPGPC-ELSD），其分子量约为 1540.10±48.78 kDa。经 PMP 衍生化高效液相色谱（HPLC）分析，CF1 由甘露糖、葡萄糖醛酸、半乳糖醛酸、木糖、半乳糖、阿拉伯糖和海藻糖组成，摩尔比为 0.68∶0.59∶0.27∶0.24∶0.22∶0.67∶0.08。体外 MTT、Tunel 检测和流式细胞术检测 CF1 的抗肿瘤作用。结果表明，CF1 对 DU145 前列腺癌细胞具有浓度依赖性的增殖抑制作用，IC50 值为 374.11μg/mL。Tunel 检测和流式细胞术检测表明 CF1 的抗肿瘤活性与体外细胞凋亡有关。本研究提示枸杞花粉 CF1 可能是一种具有潜在应用价值的抗肿瘤功能性食品或药物。
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