Department of Cardiothoracic Surgery, Taicang Affiliated Hospital of Soochow University, the First People's Hospital of Taicang, Taicang, China.
Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China.
Neoplasma. 2018 Sep 19;65(5):664-672. doi: 10.4149/neo_2018_170713N480. Epub 2018 Sep 4.
The aim of the research was to investigate the expression of lncRNA EPB41L4A-AS2 in non-small cell lung cancer (NSCLC) and evaluate its influence on the proliferation, invasion and apoptosis of NSCLC. A total of 56 NSCLC tissues and its corresponding adjacent tissues were collected. Quantitative Reverse transcription polymerase chain reaction (qRT-PCR) was performed to evaluate the lncRNA EPB41L4A-AS2 expression level in tissues and cell lines. Proliferating cell nuclear antigen (PCNA) protein level was determined by western blot assay. CCK8 assay, EdU assay, flow cytometry (FCM) and transwell assay were performed to access cell proliferation, apoptosis and invasion. EPB41L4A-AS2 expression was significantly downregulated in cancer tissues and cells compared with the adjacent tissues and normal cells (P<0.05). After cells were transfected with pcDNA3.1-EPB41L4A-AS2, cell viability and PCNA protein level was decreased, and cells were arrested in the G0/G1 phase with higher apoptosis rate. Transwell assay showed that over-expressed EPB41L4A-AS2 could reduce cells invasion ability. Expression of low levels of EPB41L4A-AS2 is associated with poor survival in NSCLC and the over-expression of lncRNA EPB41L4A-AS2 inhibits NSCLC cell proliferation, invasion and promote cell apoptosis.
这项研究的目的是探究长链非编码 RNA EPB41L4A-AS2 在非小细胞肺癌（NSCLC）中的表达，并评估其对 NSCLC 细胞增殖、侵袭和凋亡的影响。共收集了 56 份 NSCLC 组织及其相应的相邻组织。采用定量逆转录聚合酶链反应（qRT-PCR）检测组织和细胞系中 lncRNA EPB41L4A-AS2 的表达水平。采用蛋白质印迹法检测增殖细胞核抗原（PCNA）蛋白水平。通过 CCK8 assay、EdU assay、流式细胞术（FCM）和 Transwell assay 评估细胞增殖、凋亡和侵袭。与相邻组织和正常细胞相比，癌组织和细胞中 lncRNA EPB41L4A-AS2 的表达明显下调（P<0.05）。转染 pcDNA3.1-EPB41L4A-AS2 后，细胞活力和 PCNA 蛋白水平降低，细胞停滞在 G0/G1 期，凋亡率升高。Transwell assay 显示，过表达 EPB41L4A-AS2 可降低细胞侵袭能力。EPB41L4A-AS2 低表达与 NSCLC 患者的不良预后相关，过表达 lncRNA EPB41L4A-AS2 可抑制 NSCLC 细胞增殖、侵袭并促进细胞凋亡。
