Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Bangkok Noi Road, Bangkok Noi, Bangkok, 10700, Thailand.
Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
BMC Complement Altern Med. 2018 Sep 24;18(1):258. doi: 10.1186/s12906-018-2327-1.
For decades, various cardiovascular symptoms have been relieved by the use of Ya-Hom Navakot, which is a formulation comprising 54 herbal medicines. The Thailand Ministry of Public Health listed Ya-Hom Navakot's nine active principle and nomenclative herbal ingredients and termed them 'Phikud Navakot' (PN). Several reports have confirmed that PN has cardiovascular benefits similar to Ya-Hom Navakot. However, whether PN facilitates lipid-lowering activity remains unclear.
The present study investigated an in vitro model for examining the gene expression levels of 3-hydroxyl-3-methylglutaryl-CoA reductase (HMGCR) and low-density lipoprotein receptor (LDL-R) in HepG2 cells using qRT-PCR. The ethanol and water extractions of Ya-Hom Navakot, PN and Ya-Hom Navakot without PN were compared.
One mg/ml of both NYEF and NYWF were found to significantly lower cholesterol by either the up-regulation of LDL-R or down-regulation of HMGCR compared with negative controls and 1 mg/ml simvastatin (p < 0.05). PNEF also up-regulated LDL-R gene expression, even more than NYEF (p < 0.05). In addition, the ethanol and water extracts of PN significantly down-regulated HMGCR gene expression compared with those of Ya-Hom Navakot without PN (p < 0.05).
The use of Ya-Hom Navakot or PN may provide an alternative treatment to lower cholesterol through HMGCR gene inhibition and LDL-R gene enhancement.
几十年来，Ya-Hom Navakot 配方中包含 54 种草药，可缓解各种心血管症状。泰国公共卫生部列出了 Ya-Hom Navakot 的九种活性原则和命名草药成分，并将其命名为“Phikud Navakot”（PN）。有几项报告证实，PN 具有与 Ya-Hom Navakot 相似的心血管益处。然而，PN 是否促进降脂活性尚不清楚。
本研究通过 qRT-PCR 研究了 HepG2 细胞中 3-羟基-3-甲基戊二酰辅酶 A 还原酶（HMGCR）和低密度脂蛋白受体（LDL-R）基因表达水平的体外模型。比较了 Ya-Hom Navakot、PN 和不含 PN 的 Ya-Hom Navakot 的乙醇和水提取物。
与阴性对照和 1mg/ml 辛伐他汀相比，1mg/ml 的 NYEF 和 NYWF 均通过上调 LDL-R 或下调 HMGCR 显著降低胆固醇（p<0.05）。PNEF 甚至比 NYEF 更能上调 LDL-R 基因表达（p<0.05）。此外，PN 的乙醇和水提取物与不含 PN 的 Ya-Hom Navakot 相比，明显下调 HMGCR 基因表达（p<0.05）。
使用 Ya-Hom Navakot 或 PN 可能通过抑制 HMGCR 基因和增强 LDL-R 基因来提供一种降低胆固醇的替代治疗方法。
