Department of Radiology, Sapporo Medical University School of Medicine, Japan.
Department of Radiology, Sapporo City General Hospital, Japan.
Radiother Oncol. 2018 Nov;129(2):409-414. doi: 10.1016/j.radonc.2018.08.023. Epub 2018 Sep 21.
To investigate influences of proteins involved with tumor immunity on outcomes of radiotherapy for oropharyngeal squamous cell carcinoma (OPSCC).
We performed immunohistochemical staining to examine expressions of p16 and proteins involved with tumor immunity in 92 OPSCC patients treated with radiotherapy.
Patients with abundant infiltrating CD8-positive cells had the significantly better overall survival (OS) rate than patients with fewer CD8-positive cells (p = 0.026). Patients with higher PD-L1 expression in tumor cells (TC 1-3) had a better outcome than those with low PD-L1 expression in tumor cells (TC 0) for both OS (p = 0.019) and progression-free survival (PFS) rate (p = 0.032). Patients with high PD-L1 expression in infiltrating immune cells (IC 3) showed significantly better OS (p = 0.009) and PFS (p = 0.011) than those with low PD-L1 expression (IC 0-2). Patients with p16-negative and IC 3 showed similar OS to patients with p16-positive and IC 0-2. P16-positive tumors had a significantly higher CD8-positive cell infiltration and PD-L1 expression in tumor cells than p16-negative tumors.
In addition to tumor p16 expression, PD-L1 expression in TC and IC can be useful for predicting the response of OPSCC to radiotherapy.
研究肿瘤免疫相关蛋白对口腔鳞状细胞癌（OPSCC）放疗结果的影响。
我们对 92 例接受放疗的 OPSCC 患者进行了免疫组织化学染色，以检测 p16 和肿瘤免疫相关蛋白的表达。
与 CD8 阳性细胞浸润较少的患者相比，CD8 阳性细胞浸润丰富的患者总生存（OS）率显著更好（p=0.026）。肿瘤细胞（TC 1-3）中 PD-L1 表达较高的患者，其 OS（p=0.019）和无进展生存（PFS）率（p=0.032）均优于肿瘤细胞中 PD-L1 表达较低的患者。浸润免疫细胞（IC 3）中 PD-L1 表达较高的患者，OS（p=0.009）和 PFS（p=0.011）均显著优于 PD-L1 表达较低的患者（IC 0-2）。p16 阴性且 IC 3 的患者的 OS 与 p16 阳性且 IC 0-2 的患者相似。p16 阳性肿瘤的 CD8 阳性细胞浸润和肿瘤细胞 PD-L1 表达均显著高于 p16 阴性肿瘤。
除了肿瘤 p16 表达外，TC 和 IC 中的 PD-L1 表达也可用于预测 OPSCC 对放疗的反应。
