采用免疫层析 RESIST-4 O.K.N.V. 检测法检测血培养阳性标本中产碳青霉烯酶肠杆菌科细菌。
Detection of Carbapenemase-Producing Enterobacteriaceae in Positive Blood Culture Using an Immunochromatographic RESIST-4 O.K.N.V. Assay.
机构信息
IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, AP-HP, Service de Microbiologie, Hôpital Robert-Debré, AP-HP, Paris, France.
Service de Microbiologie, Centre National de Référence Associé Escherichia coli, Hôpital Robert-Debré, AP-HP, Paris, France.
出版信息
Antimicrob Agents Chemother. 2018 Nov 26;62(12). doi: 10.1128/AAC.01828-18. Print 2018 Dec.
Abstract
We evaluated the performance of the RESIST-4 O.K.N.V. assay (Coris) with 98 isolates to detect OXA-48-like and KPC-, NDM-, and VIM-type carbapenemases directly on positive human blood cultures. OXA-48-like and KPC-type isolates were correctly detected, but the detection of NDM- and VIM-type carbapenemases was weak and variable. We show that repeating the test on a 4-h subculture improves the detection of NDM- and VIM-type carbapenemases to 100%.
摘要
我们用 98 株分离株评估了 RESIST-4 O.K.N.V. 检测（Coris）在阳性血培养物上直接检测 OXA-48 样、KPC-、NDM-和 VIM 型碳青霉烯酶的性能。OXA-48 样和 KPC 型分离株被正确检测到，但 NDM-和 VIM 型碳青霉烯酶的检测较弱且不稳定。我们表明，在 4 小时的亚培养物上重复测试可将 NDM-和 VIM 型碳青霉烯酶的检测提高到 100%。
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