Baer Daria, Azrad Maya, Saleh Nora, Peretz Avi
The Azrieli Faculty of Medicine, Bar-Ilan University, Safed 1311502, Israel.
Clinical Microbiology Laboratory, The Baruch Padeh Medical Center, Poriya, Tiberias 1528001, Israel.
Life (Basel). 2021 Feb 14;11(2):145. doi: 10.3390/life11020145.
Bacteremia leading to sepsis and organ dysfunction is a life-threatening situation, leading to death of up to one fourth of the infected individuals around the world. One major challenge in the treatment of sepsis is the rising prevalence of antibiotic resistant bacteria, such as carbapenem-resistant Enterobacterales (CRE). In recent years, several molecular assays have been developed for the detection of CRE mechanisms, enabling rapid results reporting. We evaluated the performance of the NG-Test CARBA 5 (NG Biotech) kit in detection of CRE in simulated blood cultures. Carbapenemase-producing (CP) CRE isolates ( = 38) and non-carbapenemase CRE (Non-CP) isolates ( = 10), previously identified using the routine methods practiced at the clinical microbiology laboratory of the Baruch Padeh Medical Center, Israel, were used in this analysis. Variable concentrations of the bacterial isolates were added to a suspension composed of human blood and saline, simulating the composition of a blood culture. Samples were then transferred to an anaerobic blood culture bottle and later tested with the NG-Test CARBA 5 (NG Biotech) kit, that identifies the CRE mechanism within 15 min. The NG-Test CARBA 5 kit correctly identified 43 samples (89.5%). The sensitivity and specificity of the kits were 86.8% and 100%, respectively. In conclusion, the NG-Test CARBA 5 kit is a reliable and accessible tool for the rapid diagnosis of CRE bloodstream infections.
菌血症导致脓毒症和器官功能障碍是一种危及生命的情况，在全球范围内导致多达四分之一的受感染个体死亡。脓毒症治疗中的一个主要挑战是抗生素耐药菌的患病率不断上升，例如耐碳青霉烯类肠杆菌科细菌（CRE）。近年来，已经开发了几种分子检测方法来检测CRE机制，能够快速报告结果。我们评估了NG-Test CARBA 5（NG生物技术公司）试剂盒在模拟血培养中检测CRE的性能。本分析使用了之前在以色列巴鲁克·帕德赫医疗中心临床微生物实验室采用常规方法鉴定的产碳青霉烯酶（CP）的CRE分离株（n = 38）和非产碳青霉烯酶的CRE（非CP）分离株（n = 10）。将不同浓度的细菌分离株添加到由人血和盐水组成的悬浮液中，模拟血培养的成分。然后将样本转移到厌氧血培养瓶中，随后用NG-Test CARBA 5（NG生物技术公司）试剂盒进行检测，该试剂盒可在15分钟内鉴定出CRE机制。NG-Test CARBA 5试剂盒正确鉴定了43个样本（89.5%）。该试剂盒的灵敏度和特异性分别为86.8%和100%。总之，NG-Test CARBA 5试剂盒是快速诊断CRE血流感染的可靠且易用的工具。
