Yurgel Maria E, Shah Kreesha D, Brown Elizabeth B, Burns Carter, Bennick Ryan A, DiAngelo Justin R, Keene Alex C
Department of Biological Sciences, Florida Atlantic University, Jupiter, FL.
Division of Science, Pennsylvania State University Berks, Reading, PA.
G3 (Bethesda). 2018 Nov 6;8(11):3385-3395. doi: 10.1534/g3.118.200554.
Metabolic state is a potent modulator of sleep and circadian behavior, and animals acutely modulate their sleep in accordance with internal energy stores and food availability. Across phyla, hormones secreted from adipose tissue act in the brain to control neural physiology and behavior to modulate sleep and metabolic state. Growing evidence suggests the fat body is a critical regulator of complex behaviors, but little is known about the genes that function within the fat body to regulate sleep. To identify molecular factors functioning in non-neuronal tissues to regulate sleep, we performed an RNAi screen selectively knocking down genes in the fat body. We found that knockdown of /), a highly conserved gene involved the biosynthesis of purines, sleep regulation and energy stores. Flies heterozygous for multiple mutations are also short sleepers and this effect is partially rescued by restoring to the fat body. Targeted knockdown of in the fat body does not alter arousal threshold or the homeostatic response to sleep deprivation, suggesting a specific role in modulating baseline sleep duration. Together, these findings suggest functions within the fat body to promote both sleep and energy storage, providing a functional link between these processes.
代谢状态是睡眠和昼夜节律行为的有力调节因子，动物会根据体内能量储备和食物供应情况敏锐地调节自身睡眠。在整个动物界，脂肪组织分泌的激素作用于大脑，控制神经生理和行为，从而调节睡眠和代谢状态。越来越多的证据表明，脂肪体是复杂行为的关键调节因子，但对于脂肪体内调节睡眠的基因却知之甚少。为了确定在非神经组织中发挥作用以调节睡眠的分子因子，我们进行了一项RNA干扰筛选，选择性地敲低脂肪体中的基因。我们发现，敲低 /），一个参与嘌呤生物合成、睡眠调节和能量储备的高度保守基因。多个突变的杂合子果蝇也是短睡眠者，通过将 恢复到脂肪体中，这种效应部分得到挽救。在脂肪体中靶向敲低 不会改变觉醒阈值或对睡眠剥夺的稳态反应，表明其在调节基线睡眠时间方面具有特定作用。总之，这些发现表明 在脂肪体内发挥作用，促进睡眠和能量储存，为这些过程之间提供了功能联系。
