Beard Breanna, Bohn Abigail, Opoola Mubaraq, Hwangbo Dae-Sung
Department of Biology, University of Louisville, Louisville, Kentucky, United States.
MicroPubl Biol. 2024 Jun 30;2024. doi: 10.17912/micropub.biology.001200. eCollection 2024.
Sleep and feeding are conserved behaviors across many taxa of the animal kingdom and are essential for an organism's survival and fitness. Although has been used to study these behaviors for decades, concurrent measurement of these two behaviors in the same flies on solid media has been a challenge. Here, we report Con-DAM, which enables simultaneous quantification of food intake and sleep/activity at the single fly resolution. Since Con-DAM integrates the Con-Ex (Consumption-Excretion) assay and the DAM (Drosophila Activity Monitor), two widely used tools to quantify food consumption and sleep/activity in flies into a single unit, we expect Con-DAM to serve as an easy method for various purposes that require quantifying food consumption and sleep/activity in the same individual flies.
睡眠和进食是动物界许多分类群中保守的行为，对生物体的生存和健康至关重要。尽管数十年来一直使用[具体方法]来研究这些行为，但在固体培养基上对同一果蝇同时测量这两种行为一直是一项挑战。在这里，我们报告了Con-DAM，它能够在单只果蝇分辨率下同时量化食物摄入量和睡眠/活动量。由于Con-DAM将Con-Ex（消耗-排泄）测定法和DAM（果蝇活动监测器）这两种广泛用于量化果蝇食物消耗和睡眠/活动的工具整合到一个单元中，我们期望Con-DAM能作为一种简便方法，用于各种需要量化同一果蝇个体食物消耗和睡眠/活动的目的。
