Bouhamed Radia, Bouayad Leila, Messad Sara, Zenia Safia, Naïm Malek, Hamdi Taha-Mossadak
Laboratory of Food Hygiene and Quality Insurance System, High National Veterinary School, Rue Issad Abbes, 16111 El Alia, Oued Smar, Algiers, Algeria.
Department of Microbiology, Central Military Hospital, 16050 Kouba, Algiers, Algeria.
Vet World. 2018 Aug;11(8):1074-1081. doi: 10.14202/vetworld.2018.1074-1081. Epub 2018 Aug 7.
Sources of contamination, prevalence, and antimicrobial susceptibility of thermophilic isolated from turkey samples were determined.
A total of 300 samples were collected from 3 farms (fecal droppings) and 4 poultry slaughterhouses (neck skins and ceca) located in the middle area of Algeria (Algiers, Boumerdès, and Bouira). After detection, an antibiogram was realized only for slaughterhouses samples.
Samples from cecum (90.0%, 90/100; 95% confidence interval (CI)=84.1-95.9%), fecal dropping (68.0%, 68/100; 95% CI=58.9-77.1%), and neck skin (55.0%, 55/100; 95% CI=45.2-64.8%) were positive for thermophilic (p<0.05). Contamination rate of turkey carcasses was higher in modern slaughterhouse (96.7%) than in traditional slaughterhouses (37.1%) (p<0.05). Isolated strains were resistant to nalidixic acid (NA) (87.5%), tetracycline (TE) (81.3%), ciprofloxacin (CIP) (75.0%), ampicillin (AM) (65.6%), and erythromycin (25.0%) (p<0.05). 96.9% (124/128) of the isolates were multiresistant and 18 drug resistance patterns were registered. The predominant one (43.0%) was AM, NA, CIP, and TE.
Potential sources of contamination of this fastidious bacterium were noticed in farms and slaughterhouses. Modern slaughterhouse allowed contamination of turkey carcasses more than a traditional slaughterhouse. However, the scalding step could not represent a source of contamination. The most tested strains exhibited resistance to erythromycin and/or CIP. It is worrisome because these molecules are considered as first-choice antibiotics for human campylobacteriosis.
确定从火鸡样本中分离出的嗜热菌的污染来源、流行情况及抗菌药敏性。
从位于阿尔及利亚中部地区（阿尔及尔、布迈尔德和布伊拉）的3个农场（粪便）和4个家禽屠宰场（颈部皮肤和盲肠）共采集300份样本。检测后，仅对屠宰场样本进行了药敏试验。
盲肠样本（90.0%，90/100；95%置信区间（CI）=84.1 - 95.9%）、粪便样本（68.0%，68/100；95% CI=58.9 - 77.1%）和颈部皮肤样本（55.0%，55/100；95% CI=45.2 - 64.8%）的嗜热菌检测呈阳性（p<0.05）。现代屠宰场中火鸡胴体的污染率（96.7%）高于传统屠宰场（37.1%）（p<0.05）。分离菌株对萘啶酸（NA）（87.5%）、四环素（TE）（81.3%）、环丙沙星（CIP）（75.0%）、氨苄西林（AM）（65.6%）和红霉素（25.0%）耐药（p<0.05）。96.9%（124/128）的分离菌株具有多重耐药性，共记录到18种耐药模式。最主要的模式（43.0%）是AM、NA、CIP和TE。
在农场和屠宰场发现了这种苛求菌的潜在污染来源。现代屠宰场比传统屠宰场更容易导致火鸡胴体受到污染。然而，烫毛步骤并非污染来源。大多数受试菌株对红霉素和/或环丙沙星耐药。这令人担忧，因为这些药物被视为治疗人类弯曲菌病的首选抗生素。
