Sierra-Arguello Yuli M, Perdoncini G, Morgan R B, Salle C T P, Moraes H L S, Gomes Marcos J P, do Nascimento Vladimir Pinheiro
a Poultry Diagnostic and Research Centre (CDPA), Faculty of Veterinary Medicine , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
b Laboratory of Veterinary Bacteriology (LABACVET) , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
Avian Pathol. 2016;45(1):66-72. doi: 10.1080/03079457.2015.1120272.
Campylobacter jejuni is recognized as a leading cause of acute bacterial gastroenteritis in humans. The over-use of antimicrobials in the human population and in animal husbandry has led to an increase in antimicrobial-resistant infections, particularly with fluoroquinolones and macrolides. The aim of the present study was to provide information of the current status of antimicrobial resistance patterns in Campylobacter jejuni from poultry sources. Fifty strains were recovered from broiler slaughterhouses in Rio Grande do Sul state, Brazil, 2012. The strains were investigated for antimicrobial susceptibility against three agents (ciprofloxacin, nalidixic acid and erythromycin) by minimal inhibitory concentrations. The strains were analysed by polymerase chain reaction-restriction fragment length polymorphism for detection of the Thr-86 mutation that confers resistance to ciprofloxacin. In addition, all the strains were tested for the presence of efflux systems (cmeB gene) conferring antimicrobial resistance. The minimum inhibitory concentrations results showed that 98% of isolates were sensitive to erythromycin and most isolates were resistant to ciprofloxacin (94%) and nalidixic acid (90%). A complete correlation was observed between the minimum inhibitory concentrations and PCR-RFLP assay. Finally, the cmeB gene that is responsible for multidrug resistance was detected in 16 isolates out the 50 strains (32%).
空肠弯曲菌被认为是人类急性细菌性肠胃炎的主要病因。在人类群体和畜牧业中过度使用抗菌药物已导致抗菌药物耐药性感染增加，尤其是对氟喹诺酮类和大环内酯类药物的耐药性。本研究的目的是提供来自家禽源的空肠弯曲菌抗菌药物耐药模式的当前状况信息。2012年，从巴西南里奥格兰德州的肉鸡屠宰场分离出50株菌株。通过最低抑菌浓度对这些菌株进行了针对三种药物（环丙沙星、萘啶酸和红霉素）的抗菌药物敏感性研究。通过聚合酶链反应-限制性片段长度多态性分析这些菌株，以检测赋予环丙沙星耐药性的苏氨酸-86突变。此外，检测了所有菌株中赋予抗菌药物耐药性的外排系统（cmeB基因）的存在情况。最低抑菌浓度结果显示，98%的分离株对红霉素敏感，大多数分离株对环丙沙星（94%）和萘啶酸（90%）耐药。在最低抑菌浓度和聚合酶链反应-限制性片段长度多态性分析之间观察到完全相关性。最后，在50株菌株中的16株（32%）中检测到了负责多重耐药性的cmeB基因。
