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来自假单胞菌属菌株B13的3-氯苯甲酸降解基因的分子克隆与表达

Molecular cloning and expression of the 3-chlorobenzoate-degrading genes from Pseudomonas sp. strain B13.

作者信息

Weisshaar M P, Franklin F C, Reineke W

出版信息

J Bacteriol. 1987 Jan;169(1):394-402. doi: 10.1128/jb.169.1.394-402.1987.

DOI:10.1128/jb.169.1.394-402.1987
PMID:3025183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211780/
Abstract

The genes specifying the utilization of 3-chlorobenzoate by Pseudomonas sp. strain B13 WR1 have been cloned by using a broad-host-range cosmid cloning system. Analysis of the catabolic products of the enzymatic reactions encoded by two hybrid cosmids, pMW65 and pMW90, by thin-layer and high-performance liquid chromatography demonstrated that both encoded the genes for the complete catabolism of 3-chlorobenzoate. Physical analysis of one of the cosmid derivatives, pMW65, by restriction endonuclease mapping and subcloning demonstrated that the pathway genes are encoded on a fragment no larger than 11 kilobases.

摘要

利用广宿主范围黏粒克隆系统，已克隆出假单胞菌属菌株B13 WR1中指定利用3 - 氯苯甲酸的基因。通过薄层色谱法和高效液相色谱法对两个杂种黏粒pMW65和pMW90所编码的酶促反应的分解代谢产物进行分析，结果表明二者均编码3 - 氯苯甲酸完全分解代谢的基因。通过限制性内切酶图谱分析和亚克隆对其中一个黏粒衍生物pMW65进行物理分析，结果表明该途径基因位于一个不大于11千碱基的片段上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5668/211780/9a160b516e22/jbacter00191-0416-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5668/211780/7db8ba1682be/jbacter00191-0415-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5668/211780/9a160b516e22/jbacter00191-0416-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5668/211780/7db8ba1682be/jbacter00191-0415-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5668/211780/9a160b516e22/jbacter00191-0416-a.jpg

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