Chatterjee D K, Chakrabarty A M
J Bacteriol. 1983 Jan;153(1):532-4. doi: 10.1128/jb.153.1.532-534.1983.
Two chlorobenzoate-degradative plasmids were studied by the hybridization of the restriction endonuclease-generated fragments of one plasmid after transfer to a nitrocellulose filter with nick-translated radioactive DNA of the other plasmid as a probe. Two strains harboring the 3-chlorobenzoic acid-degradative plasmids were isolated in two different parts of the world at two different times. The plasmids are now found to be closely related to each other by hybridization studies. The chlorobenzoate-degradative plasmid from Pseudomonas sp. strain B13 (termed pB13) has a 6-kilobase deletion but otherwise is homologous with previously described plasmid pAC25.
通过将一种质粒经限制性内切酶产生的片段转移至硝酸纤维素滤膜上，并用另一种质粒经缺口平移产生的放射性DNA作为探针进行杂交，对两种氯苯甲酸降解质粒进行了研究。在世界上两个不同地区、两个不同时间分离出了两株携带3 - 氯苯甲酸降解质粒的菌株。通过杂交研究发现，这两种质粒现在彼此密切相关。来自假单胞菌属菌株B13的氯苯甲酸降解质粒（称为pB13）有一个6千碱基的缺失，但在其他方面与先前描述的质粒pAC25同源。
