恶臭假单胞菌中萘代谢基因的克隆
Cloning of genes for naphthalene metabolism in Pseudomonas putida.
作者信息
Grund A D, Gunsalus I C
出版信息
J Bacteriol. 1983 Oct;156(1):89-94. doi: 10.1128/jb.156.1.89-94.1983.
Abstract
Plasmid pIG7 DNA cloned in Pseudomonas putida with the broad-host-range vectors pRK290 and pKT240 expresses the genes encoding nephthalene oxidation in the presence of the intermediate substrate, salicylate, or the gratuitous inducer, anthranilate. Two operons, nahAF and nahGK, cloned from the EcoRI fragment A (25 kilobases) are under wild-type regulation by the nahR locus. Deletion plasmids provide a restriction map of both operons. Double transformants containing structural and regulatory cistron nahR in trans are used to demonstrate positive control of expression.
摘要
用广宿主范围载体pRK290和pKT240克隆于恶臭假单胞菌中的质粒pIG7 DNA，在中间底物水杨酸盐或 gratuitous诱导剂邻氨基苯甲酸存在的情况下，表达编码萘氧化的基因。从EcoRI片段A（25千碱基）克隆的两个操纵子nahAF和nahGK受nahR基因座的野生型调控。缺失质粒提供了两个操纵子的限制性图谱。含有反式结构和顺式调控顺反子nahR的双转化体用于证明表达的正调控。
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