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Cloning of genes for naphthalene metabolism in Pseudomonas putida.恶臭假单胞菌中萘代谢基因的克隆
J Bacteriol. 1983 Oct;156(1):89-94. doi: 10.1128/jb.156.1.89-94.1983.
2
Cloning and expression in Escherichia coli of the naphthalene degradation genes from plasmid NAH7.来自质粒NAH7的萘降解基因在大肠杆菌中的克隆与表达。
J Bacteriol. 1983 Feb;153(2):822-9. doi: 10.1128/jb.153.2.822-829.1983.
3
Regulation of the nah and sal operons of plasmid NAH7: evidence for a new function in nahR.质粒NAH7的nah和sal操纵子的调控：nahR新功能的证据
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Transcriptional control of the nah and sal hydrocarbon-degradation operons by the nahR gene product.nahR基因产物对nah和sal碳氢化合物降解操纵子的转录控制。
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Regulation of naphthalene catabolic genes of plasmid NAH7.质粒NAH7萘分解代谢基因的调控
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[Cloning of Pseudomonas putida genes responsible for the primary stages of oxidation of naphthalene in Escherichia coli cells].[负责萘在大肠杆菌细胞中初级氧化阶段的恶臭假单胞菌基因的克隆]
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Homology between nucleotide sequences of promoter regions of nah and sal operons of NAH7 plasmid of Pseudomonas putida.恶臭假单胞菌NAH7质粒中nah和sal操纵子启动子区域核苷酸序列之间的同源性。
Proc Natl Acad Sci U S A. 1986 Jan;83(2):369-73. doi: 10.1073/pnas.83.2.369.

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Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.恶臭假单胞菌TOL质粒pWWO的分子与功能分析及整个调控型芳香环间位裂解途径基因的克隆
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Construction of a partial diploid for the degradative pathway encoded by the TOL plasmid (pWWO) from Pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyIR gene.构建恶臭假单胞菌mt-2的TOL质粒（pWWO）编码的降解途径的部分二倍体：xyIR基因正向调控性质的证据。
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Cloning and expression in Escherichia coli of the naphthalene degradation genes from plasmid NAH7.来自质粒NAH7的萘降解基因在大肠杆菌中的克隆与表达。
J Bacteriol. 1983 Feb;153(2):822-9. doi: 10.1128/jb.153.2.822-829.1983.
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Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.专用质粒克隆载体。II. 广泛宿主范围、高拷贝数、源自RSF1010的载体以及用于假单胞菌基因克隆的宿主-载体系统。
Gene. 1981 Dec;16(1-3):237-47. doi: 10.1016/0378-1119(81)90080-9.
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Plasmid gene organization: naphthalene/salicylate oxidation.质粒基因组织：萘/水杨酸盐氧化
Proc Natl Acad Sci U S A. 1982 Feb;79(3):874-8. doi: 10.1073/pnas.79.3.874.
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Molecular cloning of gene xylS of the TOL plasmid: evidence for positive regulation of the xylDEGF operon by xylS.TOL 质粒 xylS 基因的分子克隆：xylS 对 xylDEGF 操纵子正向调控的证据
J Bacteriol. 1981 Nov;148(2):413-8. doi: 10.1128/jb.148.2.413-418.1981.
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Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida.编码恶臭假单胞菌萘氧化早期酶的可传递质粒
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恶臭假单胞菌中萘代谢基因的克隆

Cloning of genes for naphthalene metabolism in Pseudomonas putida.

作者信息

Grund A D, Gunsalus I C

出版信息

J Bacteriol. 1983 Oct;156(1):89-94. doi: 10.1128/jb.156.1.89-94.1983.

DOI:10.1128/jb.156.1.89-94.1983
PMID:6311809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215054/
Abstract

Plasmid pIG7 DNA cloned in Pseudomonas putida with the broad-host-range vectors pRK290 and pKT240 expresses the genes encoding nephthalene oxidation in the presence of the intermediate substrate, salicylate, or the gratuitous inducer, anthranilate. Two operons, nahAF and nahGK, cloned from the EcoRI fragment A (25 kilobases) are under wild-type regulation by the nahR locus. Deletion plasmids provide a restriction map of both operons. Double transformants containing structural and regulatory cistron nahR in trans are used to demonstrate positive control of expression.

摘要

用广宿主范围载体pRK290和pKT240克隆于恶臭假单胞菌中的质粒pIG7 DNA，在中间底物水杨酸盐或 gratuitous诱导剂邻氨基苯甲酸存在的情况下，表达编码萘氧化的基因。从EcoRI片段A（25千碱基）克隆的两个操纵子nahAF和nahGK受nahR基因座的野生型调控。缺失质粒提供了两个操纵子的限制性图谱。含有反式结构和顺式调控顺反子nahR的双转化体用于证明表达的正调控。