a Department of Maxillofacial Surgery , Sosnowiec , Poland.
b Division of Sports Medicine, Department of Physiotherapy, Faculty of Health Sciences , Wroclaw Medical University , Wroclaw , Poland.
Platelets. 2019;30(6):728-736. doi: 10.1080/09537104.2018.1513472. Epub 2018 Sep 25.
The current literature suggests that the antibacterial effect of leukocyte- and platelet-rich plasma (L-PRP) is directly related to platelet and leukocyte concentrations. The aim of this study was twofold: first, to evaluate the antimicrobial effect of L-PRP against selected bacterial strains , and second, to correlate this effect with leukocyte and platelet content in the final concentration. Blood was collected from 20 healthy males, and L-PRP, acellular plasma (AP), and autologous thrombin were consecutively prepared. Flow cytometry analysis of the blood, L-PRP, and AP was performed. The L-PRP gel, liquid L-PRP, and thrombin samples were tested for their antibacterial properties against seven selected bacterial strains using the Kirby-Bauer disk-diffusion method. There was notable antimicrobial activity against selected bacterial strains. No statistically significant correlations between antimicrobial activities and the platelet concentration in L-PRP were observed. Statistically significant positive correlations between selected leukocyte subtypes and antimicrobial activity were noted. A negative correlation was found between elevated monocyte count and antimicrobial activity of L-PRP against one bacterial strain studied. L-PRP possesses antimicrobial activity and can be potentially useful in the fight against certain postoperative infections. The bactericidal effect of L-PRP is caused by leukocytes, and there exists a relationship among selected leukocyte subtypes and L-PRP antimicrobial activity.
目前的文献表明，富含白细胞和血小板的血浆（L-PRP）的抗菌作用与血小板和白细胞的浓度直接相关。本研究的目的有两个：首先，评估 L-PRP 对选定细菌菌株的抗菌效果；其次，将这种效果与最终浓度中的白细胞和血小板含量相关联。从 20 名健康男性中采集血液，连续制备 L-PRP、无细胞血浆（AP）和自体凝血酶。对血液、L-PRP 和 AP 进行流式细胞术分析。使用 Kirby-Bauer 圆盘扩散法测试 L-PRP 凝胶、液体 L-PRP 和凝血酶样品对七种选定细菌菌株的抗菌特性。对选定的细菌菌株具有显著的抗菌活性。未观察到 L-PRP 中的血小板浓度与抗菌活性之间存在统计学显著相关性。观察到选定的白细胞亚型与抗菌活性之间存在统计学显著正相关。发现单核细胞计数升高与研究中一种细菌的 L-PRP 抗菌活性之间存在负相关。L-PRP 具有抗菌活性，可潜在用于对抗某些术后感染。L-PRP 的杀菌作用是由白细胞引起的，并且在选定的白细胞亚型与 L-PRP 抗菌活性之间存在关系。
