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Appl Environ Microbiol. 1998 Apr;64(4):1580-3. doi: 10.1128/AEM.64.4.1580-1583.1998.
2
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3
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Microbiology (Reading). 1998 Sep;144 ( Pt 9):2441-2448. doi: 10.1099/00221287-144-9-2441.
6
Interspecies complementation in Saccharopolyspora erythraea : elucidation of the function of oleP1, oleG1 and oleG2 from the oleandomycin biosynthetic gene cluster of Streptomyces antibioticus and generation of new erythromycin derivatives.糖多孢红霉菌中的种间互补：阐明来自抗生链霉菌的竹桃霉素生物合成基因簇中oleP1、oleG1和oleG2的功能以及新红霉素衍生物的产生。
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7
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Electroporation of germinated conidia and young mycelium as an efficient transformation system for Acremonium chrysogenum.将萌发的分生孢子和幼嫩菌丝体进行电穿孔处理，作为产黄青霉的一种高效转化系统。
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Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation.Dam和Dcm甲基化可阻止基因导入巴氏梭菌NRRL B-598：电穿孔转化、接合转移和超声穿孔法的开发
Biotechnol Biofuels. 2016 Jan 20;9:14. doi: 10.1186/s13068-016-0436-y. eCollection 2016.
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Quantification of N-acetylcysteamine activated methylmalonate incorporation into polyketide biosynthesis.定量分析 N-乙酰半胱氨酸激活的丙二酰辅酶 A 掺入聚酮化合物生物合成。
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4
Ethyl-substituted erythromycin derivatives produced by directed metabolic engineering.通过定向代谢工程产生的乙基取代红霉素衍生物。
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A Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation.通过电穿孔转化瑞氏木霉 R6 和其他链霉菌的简单快速方法。
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Polyketide synthesis: prospects for hybrid antibiotics.聚酮化合物的合成：杂合抗生素的前景
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Procaryotic and eucaryotic traits of DNA methylation in spiroplasmas (mycoplasmas).螺旋体（支原体）中DNA甲基化的原核生物和真核生物特征
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Transformation of Streptomyces erythraeus.红霉素链霉菌的转化
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Properties of in vitro recombinant derivatives of pJV1, a multi-copy plasmid from Streptomyces phaeochromogenes.来自产色链霉菌的多拷贝质粒pJV1的体外重组衍生物的特性
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Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus).糖多孢红霉菌（红霉素链霉菌）中红霉素生物合成途径脱氧糖部分相关基因的克隆
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9
Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea.参与红霉菌中红霉素合成的多功能聚酮合酶中酶结构域的组织方式。
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依赖超声处理的红霉素产生菌糖多孢红霉菌的电穿孔

Sonication-dependent electroporation of the erythromycin-producing bacterium Saccharopolyspora erythraea.

作者信息

Fitzgerald N B, English R S, Lampel J S, Vanden Boom T J

机构信息

Abbott Laboratories, North Chicago, Illinois 60064, USA.

出版信息

Appl Environ Microbiol. 1998 Apr;64(4):1580-3. doi: 10.1128/AEM.64.4.1580-1583.1998.

DOI:10.1128/AEM.64.4.1580-1583.1998
PMID:9546194
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC106195/
Abstract

We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.

摘要

我们报道了一种电转化方法的开发，该方法适用于迄今为止所检测的所有糖多孢红霉菌株。通过对菌丝体悬浮液施加超声脉冲，使营养生长的菌丝体具有电转化能力。该方案为将DNA导入丝状微生物提供了一条替代途径，否则这些微生物对转化技术具有抗性。