依赖超声处理的红霉素产生菌糖多孢红霉菌的电穿孔
Sonication-dependent electroporation of the erythromycin-producing bacterium Saccharopolyspora erythraea.
作者信息
Fitzgerald N B, English R S, Lampel J S, Vanden Boom T J
机构信息
Abbott Laboratories, North Chicago, Illinois 60064, USA.
出版信息
Appl Environ Microbiol. 1998 Apr;64(4):1580-3. doi: 10.1128/AEM.64.4.1580-1583.1998.
Abstract
We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.
摘要
我们报道了一种电转化方法的开发，该方法适用于迄今为止所检测的所有糖多孢红霉菌株。通过对菌丝体悬浮液施加超声脉冲，使营养生长的菌丝体具有电转化能力。该方案为将DNA导入丝状微生物提供了一条替代途径，否则这些微生物对转化技术具有抗性。
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