禽肺病毒磷蛋白基因的序列及体外表达
Sequence and in vitro expression of the phosphoprotein gene of avian pneumovirus.
作者信息
Ling R, Davis P J, Yu Q, Wood C M, Pringle C R, Cavanagh D, Easton A J
机构信息
Department of Biological Sciences, University of Warwick, Coventry, UK.
出版信息
Virus Res. 1995 May;36(2-3):247-57. doi: 10.1016/0168-1702(95)00008-e.
Abstract
The phosphoprotein (P) gene of two subgroup A strains of avian pneumovirus comprised 855 nucleotides containing only one substantial open reading frame encoding a protein of 278 amino acids, with a predicted M(r) of 30,323. In vitro translation of P mRNA in a wheat germ system resulted in the synthesis of two polypeptides of M(r) 35,000. Comparison of the deduced P protein sequence with that of the known mammalian pneumoviruses revealed overall amino acid identities ranging from 31 to 34.5%, suggesting a distant relationship. However, there was a much higher identity (63.2-68.4%) in a region of 57 residues, which included a heptad repeat sequence.
摘要
禽肺病毒两个A亚群毒株的磷蛋白（P）基因由855个核苷酸组成，仅含有一个编码278个氨基酸的主要开放阅读框，预测相对分子质量（M(r)）为30323。在麦胚系统中对P mRNA进行体外翻译，产生了相对分子质量为35000的两种多肽。将推导的P蛋白序列与已知哺乳动物肺病毒的序列进行比较，发现总体氨基酸同一性在31%至34.5%之间，表明亲缘关系较远。然而，在一个包含七肽重复序列的57个残基区域中，同一性要高得多（63.2% - 68.4%）。
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