Li Ting-Gang, Zhang Dan-Dan, Zhou Lei, Kong Zhi-Qiang, Hussaini Adamu S, Wang Dan, Li Jun-Jiao, Short Dylan P G, Dhar Nikhilesh, Klosterman Steven J, Wang Bao-Li, Yin Chun-Mei, Subbarao Krishna V, Chen Jie-Yin, Dai Xiao-Feng
Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
Department of Plant Pathology, University of California, Davis, Davis, CA, United States.
Front Plant Sci. 2018 Sep 11;9:1266. doi: 10.3389/fpls.2018.01266. eCollection 2018.
Cysteine-rich receptor-like kinases () are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical in a genome (). Eleven of these (>30%) are located on the A06 and D06 chromosomes, and 18 consisted of 9 paralogous pairs encoded in the A and D subgenomes. Phylogenetic analysis showed that the could be classified into four broad groups, the expansion of which has probably been driven by tandem duplication. Gene expression profiling of the in resistant and susceptible cotton cultivars revealed that a phylogenetic cluster of nine of the genes were up-regulated in response to infection. Virus-induced gene silencing of each of these nine independently revealed that the silencing of was sufficient to compromise VW resistance in . GbCRK18 expression could be induced by infection or jasmonic acid, and displayed plasma membrane localization. Therefore, our expression analyses indicated that the gene family is differentially regulated in response to Verticillium infection, while gene silencing experiments revealed that in particular confers VW resistance in .
富含半胱氨酸的类受体激酶（CRKs）是植物类受体激酶中的一个大亚家族，在植物抗病性中起关键作用。然而，关于棉花中CRK基因家族及其对黄萎病（VW）的功能的了解却很缺乏，黄萎病是由大丽轮枝菌引起的一种毁灭性病害，会显著降低棉花产量。在本研究中，我们在陆地棉基因组（Gossypium hirsutum）中总共鉴定出30个典型的CRK。其中11个（>30%）位于A06和D06染色体上，18个由A和D亚基因组中编码的9对旁系同源基因组成。系统发育分析表明，CRK可分为四大类，其扩增可能是由串联重复驱动的。对抗病和感病棉花品种中CRK的基因表达谱分析表明，9个CRK基因的一个系统发育簇在响应大丽轮枝菌感染时上调。对这9个CRK中的每一个进行病毒诱导的基因沉默独立显示，GbCRK18的沉默足以损害陆地棉对黄萎病的抗性。GbCRK18的表达可由大丽轮枝菌感染或茉莉酸诱导，并显示出质膜定位。因此，我们的表达分析表明，CRK基因家族在响应黄萎病菌感染时受到差异调控，而基因沉默实验表明，特别是GbCRK18赋予陆地棉对黄萎病的抗性。
