Song Yunhao, Wang Ruigang, Wan Yongqing, Gao Jinnan, Tian Ruihua, Duan Kaihong
College of Life Science, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China.
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China.
Sheng Wu Gong Cheng Xue Bao. 2018 Sep 25;34(9):1518-1527. doi: 10.13345/j.cjb.180006.
Lactic acid bacteria and cellulose degrading bacteria play an important role in fermentation process of silage, because they can prevent the rancidity and increase the nutritive value of silage. But the propagation of lactic acid bacteria will inhibit the activity of cellulose degrading bacteria in the silage fermentation system. This problem can be solved by releasing lactic acid bacteria and cellulose degrading bacteria in different time. Therefore, we immobilized lactic acid bacteria as a microbial agent for sustained release. Firstly, the optimal balling concentration of the composite immobilized carrier and composite immobilized carrier were obtained by immobilization of blank balls and corncob adsorbed Lactobacillus plantarum S1 respectively. The best immobilization condition of L. plantarum S1 was obtained by comparing the immobilized rate and balling effect of two kinds of balls, which were embedded by sodium alginate (SA), CMC-Na and embedded-crosslinked by SA, CMC-Na, polyvinyl alcohol (PVA). The results showed that the best balling concentration was achieved by using 6% PVA+0.4% SA+0.3% CMC-Na for embedding-crosslinking and 1.2% SA+0.5% CMC-Na for direct embedding respectively. In addition, comparing with the mechanical strength and embedding rate of five kinds of immobilization process, the best immobilized process was obtained by adding of the mixture of immobilized carriers (1.2%SA+ 0.5%CMC-Na) and corncob adsorbed L. plantarum S1 slowly into 4% CaCl₂ for 24 hours. The corncob adsorption and SA embedding methodology can effectively increase the embedding efficiency of Lactobacillus plantarum S1.
乳酸菌和纤维素降解菌在青贮饲料的发酵过程中发挥着重要作用，因为它们可以防止青贮饲料酸败并提高其营养价值。但是乳酸菌的繁殖会抑制青贮饲料发酵系统中纤维素降解菌的活性。通过在不同时间释放乳酸菌和纤维素降解菌可以解决这个问题。因此，我们将乳酸菌固定化为一种用于缓释的微生物制剂。首先，分别通过空白球的固定化和玉米芯吸附植物乳杆菌S1来获得复合固定化载体的最佳造粒浓度和复合固定化载体。通过比较两种球（分别用海藻酸钠（SA）、羧甲基纤维素钠（CMC-Na）包埋以及用SA、CMC-Na、聚乙烯醇（PVA）包埋交联）的固定化率和造粒效果，获得了植物乳杆菌S1的最佳固定化条件。结果表明，分别采用6% PVA + 0.4% SA + 0.3% CMC-Na进行包埋交联和1.2% SA + 0.5% CMC-Na进行直接包埋可达到最佳造粒浓度。此外，通过比较五种固定化工艺的机械强度和包埋率，将固定化载体（1.2% SA + 0.5% CMC-Na）和玉米芯吸附的植物乳杆菌S1的混合物缓慢加入4%氯化钙中24小时，获得了最佳的固定化工艺。玉米芯吸附和SA包埋方法可以有效提高植物乳杆菌S1的包埋效率。
