黑腹果蝇RpII215基因5'区域的P因子插入位点及P因子缺失结构
Sites of P element insertion and structures of P element deletions in the 5' region of Drosophila melanogaster RpII215.
作者信息
Searles L L, Greenleaf A L, Kemp W E, Voelker R A
出版信息
Mol Cell Biol. 1986 Oct;6(10):3312-9. doi: 10.1128/mcb.6.10.3312-3319.1986.
Abstract
Several P element insertion and deletion mutations near the 5' end of Drosophila melanogaster RpII215 have been examined by nucleotide sequencing. Two different sites of P element insertion, approximately 90 nucleotides apart, have been detected in this region of the gene. Therefore, including an additional site of P element insertion within the coding region, there are at least three distinct sites of P element insertion at RpII215. Both 5' sites are within a noncoding portion of transcribed sequences. The sequences of four revertants of one P element insertion mutation (D50) indicate that the P element is either precisely deleted or internally deleted to restore RpII215 activity. Partial internal deletions of the P element result in different RpII215 activity levels, which appear to depend on the specific sequences that remain after excision.
摘要
通过核苷酸测序对黑腹果蝇RpII215 5'端附近的几个P因子插入和缺失突变进行了检测。在该基因的这一区域检测到两个不同的P因子插入位点，相距约90个核苷酸。因此，包括编码区内的另一个P因子插入位点，RpII215至少有三个不同的P因子插入位点。两个5'端位点都位于转录序列的非编码部分。一个P因子插入突变（D50）的四个回复体的序列表明，P因子要么被精确删除，要么内部删除以恢复RpII215活性。P因子的部分内部缺失导致不同的RpII215活性水平，这似乎取决于切除后剩余的特定序列。
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