1 Department of Plant Medicine and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828 Korea.
2 Dvision of Applied Life Science (BK21Plus), Gyeongsang National University.
Mol Plant Microbe Interact. 2019 Mar;32(3):306-312. doi: 10.1094/MPMI-05-18-0129-R. Epub 2019 Jan 16.
Streptomyces griseus S4-7 is representative of strains responsible for the specific soil suppressiveness of Fusarium wilt of strawberry caused by Fusarium oxysporum f. sp. fragariae. Members of the genus Streptomyces secrete diverse secondary metabolites including lantipeptides, heat-stable lanthionine-containing compounds that can exhibit antibiotic activity. In this study, a class II lantipeptide provisionally named grisin, of previously unknown biological function, was shown to inhibit F. oxysporum. The inhibitory activity of grisin distinguishes it from other class II lantipeptides from Streptomyces spp. Results of quantitative reverse transcription-polymerase chain reaction with lanM-specific primers showed that the density of grisin-producing Streptomyces spp. in the rhizosphere of strawberry was positively correlated with the number of years of monoculture and a minimum of seven years was required for development of specific soil suppressiveness to Fusarium wilt disease. We suggest that lanM can be used as a diagnostic marker of whether a soil is conducive or suppressive to the disease.
灰色链霉菌 S4-7 是引起草莓枯萎病的尖孢镰刀菌草莓专化型的特定土壤抑制性的菌株代表。链霉菌属成员分泌多种次级代谢产物，包括 lantipeptides，热稳定的含 lanthionine 的化合物，具有抗生素活性。在本研究中，一种 II 类 lantipeptide 被暂时命名为 grisin，其具有未知的生物学功能，被证明可以抑制尖孢镰刀菌。grisin 的抑制活性使其有别于来自链霉菌属的其他 II 类 lantipeptides。用 lanM 特异性引物进行的定量逆转录聚合酶链反应的结果表明，草莓根际产生 grisin 的链霉菌属的密度与单作的年限呈正相关，并且需要至少七年的时间才能对枯萎病产生特定的土壤抑制性。我们建议，lanM 可以用作土壤是否有利于或抑制疾病的诊断标记。
