Toohey M G, Morley K L, Peterson D O
Mol Cell Biol. 1986 Dec;6(12):4526-38. doi: 10.1128/mcb.6.12.4526-4538.1986.
Sets of genes under a common regulatory control in a given cell type are often differentially transcribed. The possibility that this differential transcription can be modulated by the number or strength of cis-acting regulatory sequences associated with a given gene was tested by using the glucocorticoid-responsive enhancer element associated with the mouse mammary tumor virus promoter. Results indicate that differential levels of hormone-inducible gene expression can be modulated in an additive way by the number of glucocorticoid-responsive enhancers associated with this promoter. Realization of these effects shows little preference for position of the additional elements with respect to the promoter. When sequences that bind the glucocorticoid receptor in vitro with somewhat lower affinity than the enhancer were tested, these additive effects were not detected. The results support that differential transcription of genes subject to a common regulatory control can be mediated, at least in part, by the number or strength of their associated cis-acting regulatory sequences.
在特定细胞类型中受共同调控的基因集通常会有差异转录。通过使用与小鼠乳腺肿瘤病毒启动子相关的糖皮质激素反应增强子元件，测试了这种差异转录是否可由与给定基因相关的顺式作用调控序列的数量或强度来调节。结果表明，激素诱导基因表达的差异水平可通过与该启动子相关的糖皮质激素反应增强子的数量以累加方式进行调节。这些效应的实现对额外元件相对于启动子的位置几乎没有偏好。当测试与糖皮质激素受体在体外结合亲和力略低于增强子的序列时，未检测到这些累加效应。结果支持，受共同调控的基因的差异转录至少部分可由其相关顺式作用调控序列的数量或强度介导。
