Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), Department of Bioengineering, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613 401, Tamil Nadu, India.
Centre for Biotechnology, Anna University, Chennai, 600 025, Tamil Nadu, India.
Biomed Pharmacother. 2018 Nov;107:908-917. doi: 10.1016/j.biopha.2018.08.072. Epub 2018 Aug 22.
Chitosan (CS), glycerophosphate (GP) based injectable hydrogels are explored for its implications in bone defect healing and regeneration. Both acellular and cell laden CS based hydrogels are widely investigated and improved through the inclusion of various nanoparticles, polymers and bioactive molecules. In order to improve its applicability for bone tissue repair, we developed an injectable, thermosensitive CS hydrogel containing graphene oxide (GO) and investigated its properties. The hydrogels were investigated for its porous architecture using scanning electron microscopy (SEM), swelling property, protein adsorption ability, degradation rate and exogenous biomineralization. GO addition improved the physico-chemical properties with notable betterment. The CS/GP/GO hydrogel was biocompatible to mesenchymal stem cells and they were metabolically active upon encapsulation. The hydrogel promoted osteogenic differentiation of mouse mesenchymal stem cells by upregualtion of Runt-related transcription factor 2 (Runx2), Alkaline phosphatase (ALP), Type -1 collagen (COL-1), and osteocalcin (OC) under osteogenic conditions. The hydrogel proves to be an amenable platform for carrying cells and exhibited suitable properties to be a potential candidate for bone tissue regeneration.
壳聚糖（CS）、甘油磷酸酯（GP）基可注射水凝胶因其在骨缺损愈合和再生中的应用而受到关注。无细胞和细胞负载的 CS 基水凝胶通过包含各种纳米粒子、聚合物和生物活性分子得到了广泛的研究和改进。为了提高其在骨组织修复中的适用性，我们开发了一种含有氧化石墨烯（GO）的可注射、温敏性 CS 水凝胶，并研究了其性能。使用扫描电子显微镜（SEM）研究了水凝胶的多孔结构、溶胀性能、蛋白质吸附能力、降解率和外源性生物矿化。GO 的加入改善了物理化学性质，有显著的提高。CS/GP/GO 水凝胶对间充质干细胞具有生物相容性，并且在封装后具有代谢活性。该水凝胶通过上调 Runt 相关转录因子 2（Runx2）、碱性磷酸酶（ALP）、I 型胶原（COL-1）和骨钙素（OC），在成骨条件下促进小鼠间充质干细胞的成骨分化。该水凝胶被证明是一种适合携带细胞的平台，并表现出适合作为骨组织再生的潜在候选物的特性。
