Alharbi Mona G, Al-Hindi Rashad R, Alotibi Ibrahim A, Azhari Sheren A, Farsi Reem M, Teklemariam Addisu D
Department of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Health Information Technology Department, Applied College, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Heliyon. 2023 Jan 19;9(1):e13077. doi: 10.1016/j.heliyon.2023.e13077. eCollection 2023 Jan.
Foodborne infections caused by spp. are among the most common foodborne diseases in the world. We isolated a lytic phage against extended-spectrum beta-lactam producing strain PT1 derived from chicken carcass. Results from electronmicrography indicated that phiPT1 belonged to the family, , in the order, . Phage phiPT1 was stable at temperatures from 4 °C to 60 °C and inactivated at 90 °C. phiPT1 retained a high titer from pH 4 to pH 10 for at least 1 h. Nevertheless, it displayed a significant decrease ( < 0.05) in titer at pH 11 and 12, with phage titers of 5.5 and 2.4 log PFU/mL, respectively. The latent time and burst size of phiPT1 were estimated to be 30 min and 252 PFU/infected cell, respectively. The virulence of phage phiPT1 was evaluated against Enteritidis strain PT1 at different MOIs. phiPT1 reduced proliferation relative to the negative control (MOI 0) at all MOIs ( < 0.05). However, there is no significant difference among the MOIs ( > 0.05). The phage-antibiotic combination analysis (PAS) indicated that synergism was not detected at higher phiPT1 titer (10 PFU/mL) with all tested antibiotics at all subinhibitory concentrations. However, synergistic activities were recorded at 0.25 × MIC of four tested antibiotics: cefixime, gentamicin, ciprofloxacin, and aztreonam in combination with phage at 10, 10 and 10 PFU/mL (ΣFIC ≤0.5). Synergism was detected for all antibiotics (0.1 × MIC) except meropenem and colistin in combination with phiPT1 at 10, 10 and 10 PFU/mL (ΣFIC ≤0.5). Synergism also displayed at the lowest concentrations of all antibiotics (0.01 MIC) in combination with phiPT1 at all titers except 10 PFU/mL. Such characteristic features make phiPT1 to be a potential candidate for therapeutic uses.
由[具体菌名]引起的食源性感染是世界上最常见的食源性疾病之一。我们从鸡胴体中分离出一株针对产超广谱β-内酰胺酶的[具体菌名]菌株PT1的裂解性噬菌体。电子显微镜观察结果表明，噬菌体phiPT1属于[噬菌体科名]科，[噬菌体目名]目。噬菌体phiPT1在4℃至60℃的温度范围内稳定，在90℃时失活。phiPT1在pH值为4至10时至少1小时内保持高滴度。然而，在pH值为11和12时，其滴度显著下降（P<0.05），噬菌体滴度分别为5.5和2.4 log PFU/mL。phiPT1的潜伏期和裂解量估计分别为30分钟和252 PFU/感染细胞。在不同的感染复数（MOI）下评估了噬菌体phiPT1对肠炎沙门氏菌菌株PT1的毒力。在所有感染复数下，相对于阴性对照（MOI 0），phiPT1均降低了[具体菌名]的增殖（P<0.05）。然而各感染复数之间没有显著差异（P>0.05）。噬菌体-抗生素联合分析（PAS）表明，在所有亚抑菌浓度下，当噬菌体phiPT1滴度较高（10 PFU/mL）时，与所有测试抗生素均未检测到协同作用。然而，在四种测试抗生素（头孢克肟、庆大霉素、环丙沙星和氨曲南）的0.25×最低抑菌浓度（MIC）下，与噬菌体在10、10和10 PFU/mL组合时记录到协同活性（ΣFIC≤0.5）。除美罗培南和黏菌素外，在所有抗生素（0.1×MIC）与phiPT1在10、10和10 PFU/mL组合时（ΣFIC≤0.5）均检测到协同作用。在所有抗生素最低浓度（0.01 MIC）下，与phiPT1在除10 PFU/mL外的所有滴度组合时也显示出协同作用。这些特性使phiPT1成为治疗用途的潜在候选者。
