Natural Products for Neuroprotection and Anti-Ageing Research Unit, Chulalongkorn University, Bangkok, 10330, Thailand.
College of Public Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
Sci Rep. 2023 Oct 4;13(1):16707. doi: 10.1038/s41598-023-43846-9.
Achieving healthy aging and providing protection from aging-related diseases is a major global concern. Probiotics, are a safer and more natural alternative. Moreover, identifying novel probiotics can help develop a new therapeutic approach and may help in personalized probiotic-formulations for individual's unique gut microbiome. In this study, we evaluated the benefits of our novel probiotic strains in promoting healthy aging and whether they protect against Amyloid β toxicity of Alzheimer's disease. Henceforth, we analyzed the impact of four different probiotics (Lactobacillus paracasei HII01, L. rhamnosus, L. reuteri, L. salivarius) on the lifespan extension of Caenorhabditis elegans model. Our results determine that L. paracasei HII01 provided the most positive effect on longevity and antiaging effects on C. elegans. The qPCR data and mutant-based studies indicated that L. paracasei HII01-mediated lifespan extension could be modulated by DAF-16 mediated pathway. The probiotic strains also protected the worms from the toxicity induced by β-Amyloid-expressing (Aβ) transgenic C. elegans strains, and L. paracasei HII01 provided the most significant protection. Overall, identifying novel probiotics is an important area of research that can improve health outcomes. Our study showed that L. paracasei HII01 could be considered a dietary supplement for providing healthy aging and preventing aging-related diseases.
实现健康老龄化和预防与衰老相关的疾病是一个全球性的重大关注点。益生菌是一种更安全、更自然的选择。此外，鉴定新型益生菌有助于开发新的治疗方法，并可能有助于针对个体独特的肠道微生物组进行个性化益生菌配方。在这项研究中，我们评估了我们新型益生菌菌株在促进健康老龄化方面的益处，以及它们是否能预防阿尔茨海默病的淀粉样β毒性。因此，我们分析了四种不同益生菌（副干酪乳杆菌 HII01、鼠李糖乳杆菌、雷特氏乳杆菌、唾液乳杆菌）对秀丽隐杆线虫模型寿命延长的影响。我们的结果表明，副干酪乳杆菌 HII01对长寿和抗衰老作用对秀丽隐杆线虫的影响最为积极。qPCR 数据和基于突变体的研究表明，副干酪乳杆菌 HII01 介导的寿命延长可以通过 DAF-16 介导的途径进行调节。益生菌菌株还能保护蠕虫免受β-淀粉样蛋白表达（Aβ）转基因秀丽隐杆线虫菌株诱导的毒性，副干酪乳杆菌 HII01 提供了最显著的保护。总的来说，鉴定新型益生菌是一个重要的研究领域，可以改善健康结果。我们的研究表明，副干酪乳杆菌 HII01 可以被视为一种提供健康老龄化和预防与衰老相关疾病的膳食补充剂。
