Boujnane Meryem, Zommiti Mohamed, Lesouhaitier Olivier, Ferchichi Mounir, Tahrioui Ali, Boukerb Amine M, Connil Nathalie
CBSA UR 4312, Université de Rouen Normandie, Université de Caen Normandie, Normandie Université, F-76000 Rouen, France.
Unité de Protéomique Fonctionnelle et Potentiel Nutraceutique de la Biodiversité de Tunisie, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université Tunis El-Manar, Tunis 1006, Tunisia.
Pathogens. 2025 Mar 3;14(3):244. doi: 10.3390/pathogens14030244.
is an opportunistic pathogenic bacterium, responsible for several life-threatening infections due to its multiple virulence factors and problematic multi-drug resistance, hence the necessity to find alternatives such as competitive probiotics. MZF16 is an LAB strain, isolated from traditional dried meat "Ossban", with high probiotic potential. Our study investigated the capacity of MZF16 to counteract H103 using several tests on intestinal cells (analysis of cytotoxicity, inflammation, adhesion/invasion) and on the in vivo model. The effect of MZF16 on the of the pathogen was also examined. We found that MZF16 was able to reduce H103 cytotoxicity and inflammatory activity and prevented pathogen colonization and translocation across Caco-2/TC7 cells. MZF16 also exerted an anti-virulence effect by attenuating (QS) molecules and pyoverdine production and extended lifespan. The obtained results highlight the potential of MZF16 probiotic strain as an anti- alternative and establish a basis for elucidating the mechanisms of MZF16 involved in countering virulence.
是一种机会致病菌，因其多种毒力因子和棘手的多重耐药性而导致多种危及生命的感染，因此有必要寻找如竞争性益生菌等替代物。MZF16是一株从传统肉干“Ossban”中分离出的乳酸菌菌株，具有很高的益生菌潜力。我们的研究通过对肠道细胞进行多项测试（细胞毒性、炎症、黏附/侵袭分析）以及体内模型，研究了MZF16对抗H103的能力。还检测了MZF16对病原体的影响。我们发现MZF16能够降低H103的细胞毒性和炎症活性，并防止病原体在Caco-2/TC7细胞上定植和转运。MZF16还通过减弱（群体感应）QS分子和绿脓菌素的产生发挥抗毒力作用，并延长了寿命。所得结果突出了MZF16益生菌菌株作为抗替代物的潜力，并为阐明MZF16对抗毒力的机制奠定了基础。
