Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, Pakistan.
BMC Genomics. 2018 Sep 26;19(1):712. doi: 10.1186/s12864-018-5100-4.
Aeromonas hydrophila is a potential zoonotic pathogen and primary fish pathogen. With overlapping characteristics, multiple isolates are often mislabelled and misclassified. Moreover, the potential pathogenic factors among the publicly available genomes in A. hydrophila strains of different origins have not yet been investigated.
To identify the valid strains of A. hydrophila and their pathogenic factors, we performed a pan-genomic study. It revealed that there were 13 mislabelled strains and 49 valid strains that were further verified by Average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH) and in silico multiple locus strain typing (MLST). Multiple numbers of phages were detected among the strains and among them Aeromonas phi 018 was frequently present. The diversity in type III secretion system (T3SS) and conservation of type II and type VI secretion systems (T2SS and T6SS, respectively) among all the strains are important to study for designing future strategies. The most prevalent antibiotic resistances were found to be beta-lactamase, polymyxin and colistin resistances. The comparative analyses of sequence type (ST) 251 and other ST groups revealed that there were higher numbers of virulence factors in ST-251 than in other STs group.
Publicly available genomes have 13 mislabelled organisms, and there are only 49 valid A. hydrophila strains. This valid pan-genome identifies multiple prophages that can be further utilized. Different A. hydrophila strains harbour multiple virulence factors and antibiotic resistance genes. Identification of such factors is important for designing future treatment regimes.
嗜水气单胞菌是一种潜在的人畜共患病病原体和主要的鱼类病原体。由于具有重叠的特征，许多分离株经常被错误标记和分类。此外，不同来源的嗜水气单胞菌菌株中公开基因组的潜在致病因素尚未得到研究。
为了鉴定有效的嗜水气单胞菌菌株及其致病因素，我们进行了泛基因组研究。结果表明，有 13 株是错误标记的菌株，49 株是有效的菌株，这些菌株进一步通过平均核苷酸同一性（ANI）、数字 DNA-DNA 杂交（dDDH）和体外多位点菌株分型（MLST）得到验证。在这些菌株中检测到多个噬菌体，其中嗜水气单胞菌 phi 018 经常存在。所有菌株中 III 型分泌系统（T3SS）的多样性和 II 型和 VI 型分泌系统（T2SS 和 T6SS）的保守性对于设计未来的策略非常重要。发现最常见的抗生素耐药性是β-内酰胺酶、多粘菌素和粘菌素耐药性。序列型（ST）251 和其他 ST 组的比较分析表明，ST-251 比其他 ST 组具有更高数量的毒力因子。
公开的基因组中有 13 个错误标记的生物体，只有 49 个有效的嗜水气单胞菌菌株。这个有效的泛基因组确定了多个可以进一步利用的前噬菌体。不同的嗜水气单胞菌菌株携带多种毒力因子和抗生素耐药基因。鉴定这些因素对于设计未来的治疗方案非常重要。
