Ma Tengfei, Xue Han, Piao Chungen, Liu Chengyi, Yang Mei, Bian Danran, Li Yong
Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.
Panzhihua City Academy of Agricultural and Forest Sciences, Panzhihua, China.
Front Microbiol. 2022 Apr 13;13:849695. doi: 10.3389/fmicb.2022.849695. eCollection 2022.
A novel Gram-stain-negative, aerobic, motile bacterial strain, D13-10-4-6, was isolated from the bark sample of × . The strain could grow at 15-35°C, at pH 6-10 and in 0-4% (w/v) NaCl, and the strain tested positive for oxidase and catalase activities. The main polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The main respiratory quinone was Q-10, and the predominant fatty acid was C ω7. The phylogenetic analyses showed that the strain belonged to the genus of the family Rhodobacteraceae. The family Rhodobacteraceae is an ecologically diverse group that includes bacteria from aquatic to terrestrial ecosystems. As a consequence, the classification of the family Rhodobacteraceae is difficult, not least when the early taxonomy work relied heavily on 16S rRNA gene analysis. Recently, the taxonomic status of many members of the family has been revised based on the genome analysis; however, there are still some classification conflicts due to the lack of genome sequences and parallel publication time. In this study, phylogenetic trees based on 16S rRNA gene, B gene, and 120 concatenated proteins, the average amino acid identity (AAI) and percentage of conserved proteins (POCP) have been used for the analysis of strain D13-10-4-6 and other members of 15 genera within the family to further clarify their taxonomic relationships. For the data of phylogeny, AAI, and POCP, the taxonomic proposals are (1) reclassification of as the type species of a novel genus, gen. nov., as comb. nov.; (2) reclassification of , , into a novel genus, gen. nov., as comb. nov., comb. nov., comb. nov.; (3) reclassification of into the genus as comb. nov.; (4) reclassification of , , and into the genus as comb. nov., comb. Nov., and comb. nov.; (5) reclassification of into the genus as comb. nov.; (6) classification of strain D13-10-4-6 as a novel species of the genus , for which the name sp. nov. is proposed, the type strain is D13-10-4-6 (= CFCC 12033 = KCTC 82215).
从×的树皮样本中分离出一株新型革兰氏阴性、需氧、运动性细菌菌株D13 - 10 - 4 - 6。该菌株能在15 - 35°C、pH值6 - 10和0 - 4%（w/v）NaCl条件下生长，且氧化酶和过氧化氢酶活性检测呈阳性。主要极性脂类为磷脂酰单甲基乙醇胺、二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。主要呼吸醌为Q - 10，主要脂肪酸为C ω7。系统发育分析表明，该菌株属于红杆菌科的×属。红杆菌科是一个生态多样的类群，包括从水生到陆地生态系统的细菌。因此，红杆菌科的分类很困难，尤其是早期的分类学工作严重依赖16S rRNA基因分析。最近，基于基因组分析对该科许多成员的分类地位进行了修订；然而，由于缺乏基因组序列和平行发表时间，仍然存在一些分类冲突。在本研究中，基于16S rRNA基因、B基因和120个串联蛋白构建的系统发育树、平均氨基酸同一性（AAI）和保守蛋白百分比（POCP）已用于分析菌株D13 - 10 - 4 - 6以及该科15个属的其他成员，以进一步阐明它们的分类关系。对于系统发育、AAI和POCP的数据，分类建议如下：（1）将×重新分类为一个新属×属的模式种，×新组合；（2）将×、×、×重新分类为一个新属×属，×新组合、×新组合、×新组合；（3）将×重新分类到×属，×新组合；（4）将×、×和×重新分类到×属，×新组合、×新组合和×新组合；（5）将×重新分类到×属，×新组合；（6）将菌株D13 - 10 - 4 - 6分类为×属的一个新物种，为此提出名称×新种，模式菌株为D13 - 10 - 4 - 6（= CFCC 12033 = KCTC 82215）。
