Nathans J, Hogness D S
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4851-5. doi: 10.1073/pnas.81.15.4851.
We have isolated and completely sequenced the gene encoding human rhodopsin. The coding region of the human rhodopsin gene is interrupted by four introns, which are located at positions analogous to those found in the previously characterized bovine rhodopsin gene. The amino acid sequence of human rhodopsin, deduced from the nucleotide sequence of its gene, is 348 residues long and is 93.4% homologous to that of bovine rhodopsin. Interestingly, those portions of the polypeptide chain predicted to form loops on the cytoplasmic face of rhodopsin are perfectly conserved between the human and bovine proteins.
我们已经分离出编码人类视紫红质的基因并完成了其全序列测定。人类视紫红质基因的编码区被四个内含子打断，这些内含子的位置与先前鉴定的牛视紫红质基因中的内含子位置相似。从其基因的核苷酸序列推导出来的人类视紫红质的氨基酸序列长度为348个残基，与牛视紫红质的氨基酸序列同源性为93.4%。有趣的是，预计在视紫红质细胞质面形成环的多肽链部分在人类和牛的蛋白质之间是完全保守的。
