Kristie T M, Roizman B
Proc Natl Acad Sci U S A. 1987 Jan;84(1):71-5. doi: 10.1073/pnas.84.1.71.
The herpes simplex virus 1 genes form at least five groups (alpha, beta 1, beta 2, gamma 1, and gamma 2) whose expression is coordinately regulated and sequentially ordered in a cascade fashion. In productively infected cells, the alpha genes are expressed first, and a virion protein, the alpha-trans-inducing factor (alpha-TIF), acts in trans to enhance their expression. Induction of the alpha genes by alpha-TIF requires the presence of a trans-induction cis-acting site (alpha-TIC), and one to three homologs of the alpha-TIC sequence are contained in the regulatory domains of all alpha genes. We report that small DNA fragments from regulatory domains of alpha 0, alpha 4, and alpha 27 genes containing alpha-TIC homologs formed complexes with host but not viral proteins. DNase protection studies indicated that the major host protein complex alpha-H1 detected in DNA gel retardation assays bound asymmetrically across the alpha-TIC site. All DNA fragments containing alpha-TIC homologs, but not those lacking the homolog, competed for the binding of this complex. The location of the binding site of the other host proteins is not yet known. Simian virus 40 DNA fragments containing a homolog of the alpha-TIC sequence also competed with herpes simplex virus DNA fragments carrying authentic alpha-TIC homologs for the alpha-H1 protein complex.
单纯疱疹病毒1型基因至少形成五组（α、β1、β2、γ1和γ2），其表达以级联方式进行协调调节并按顺序排列。在产生性感染的细胞中，α基因首先表达，一种病毒体蛋白，即α反式诱导因子（α-TIF），以反式作用增强其表达。α-TIF对α基因的诱导需要反式诱导顺式作用位点（α-TIC）的存在，并且所有α基因的调控域中都包含一到三个α-TIC序列的同源物。我们报道，来自α0、α4和α27基因调控域的含有α-TIC同源物的小DNA片段与宿主蛋白而非病毒蛋白形成复合物。DNA酶保护研究表明，在DNA凝胶阻滞试验中检测到的主要宿主蛋白复合物α-H1在α-TIC位点上不对称结合。所有含有α-TIC同源物的DNA片段，但不包括那些缺乏同源物的片段，都能竞争该复合物的结合。其他宿主蛋白结合位点的位置尚不清楚。含有α-TIC序列同源物的猿猴病毒40 DNA片段也能与携带真实α-TIC同源物的单纯疱疹病毒DNA片段竞争α-H1蛋白复合物的结合。
