Zhang Bohan, Sun Mengxiong, Yang Yifan, Chen Lingchao, Zou Xiang, Yang Tian, Hua Yingqi, Ji Minbiao
State Key Laboratory of Surface Physics and Department of Physics, Collaborative Innovation Center of Genetics and Development, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai 200433, China.
Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
Biomed Opt Express. 2018 May 10;9(6):2604-2613. doi: 10.1364/BOE.9.002604. eCollection 2018 Jun 1.
Two-color stimulated Raman scattering (SRS) microscopy with label-free mapping of lipid/protein distributions has shown promise in virtual histology. Despite previous demonstrations of SRS in tumor delineation and diagnosis, the speed and efficiency of the current technique requires further improvements for practical use. Here, we integrate parallel dual-phase SRS detection with strip mosaicing, which reduces the imaging time of a whole mouse brain section from 70 to 8 minutes. We further verified our method in imaging fresh human surgical tissues, showing its great potential for rapid SRS histology, especially for large scale, large quantity imaging tasks.
具有脂质/蛋白质分布无标记映射功能的双色受激拉曼散射（SRS）显微镜在虚拟组织学方面已展现出前景。尽管此前已有SRS用于肿瘤勾勒和诊断的相关演示，但当前技术的速度和效率仍需进一步改进以用于实际应用。在此，我们将并行双相SRS检测与条状拼接相结合，这将整个小鼠脑切片的成像时间从70分钟缩短至8分钟。我们还在新鲜的人类手术组织成像中验证了我们的方法，显示出其在快速SRS组织学方面的巨大潜力，特别是对于大规模、大量成像任务。
