Kim Minkyung, Hong Jinki, Shin Hyun-Joon
Center for Bionics, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, South Korea.
Biomedical Engineering, KIST School, UST, Korea University of Science and Technology, Seoul 02792, South Korea.
Biomed Opt Express. 2018 May 21;9(6):2699-2715. doi: 10.1364/BOE.9.002699. eCollection 2018 Jun 1.
We present a new fiber-bundle-based endomicroscopy system to measure the fast cerebral blood flow (CBF) velocity in blood vessels located between the surface and the deep brain of living animals. The CBF velocity is obtained by measuring the displacement of the partially overlapped red blood cell images directly, using double-pulse 532-nm laser illumination. The proposed method could measure CBF in blood vessels with diameters ranging from 4 μm to 42 μm and could measure CBF velocities up to 3.2 μm/ms for different vessel diameters at a depth of 2.1 mm from the brain surface in a living mouse.
我们展示了一种基于纤维束的新型内镜系统，用于测量活体动物大脑表面与深部之间血管中的快速脑血流（CBF）速度。通过使用双脉冲532纳米激光照明直接测量部分重叠的红细胞图像的位移来获得CBF速度。该方法可以测量直径从4μm到42μm的血管中的CBF，并且在活体小鼠中距脑表面2.1mm的深度处，对于不同血管直径可以测量高达3.2μm/ms的CBF速度。
