Diagnosis of dermatophytosis using single fungus endogenous fluorescence spectrometry.

We propose to use a single fungus endogenous fluorescence spectrometry base on a hyperspectral fluorescence microscope for the diagnosis of dermatophytosis. Dermatophyte samples, including were imaged, and the endogenous fluorescence spectrum of a single fungus was calculated. High contrast fluorescence images and endogenous fluorescence spectrum of the single fungus were used to identify the type of dermatophyte. Morphologically similar and can be distinguished using an endogenous fluorescence spectrum of the single fungus. Meanwhile, our result showed that the sensitivity and specificity of identifying were 95% and 93%, and the sensitivity and specificity of identifying were 94% and 93%.