Kamiya H, Shimizu M, Suzuki M, Inoue H, Ohtsuka E
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Nucleosides Nucleotides. 1992;11(2-4):247-60.
To evaluate the base-pairing properties and mutagenicity of deoxyxanthosine in DNA, the modified base was incorporated into a synthetic c-Ha-ras gene and a DNA transfection experiment was done. The ras gene containing deoxyxanthosine showed very high focus-forming activity. Analysis of the genes from transformants showed almost exclusively a transition of G to A. These results indicate that dTMP was preferentially incorporated at the site opposite to deoxyxanthosine, and deoxyxanthosine can induce G to A transitions in mammalian cells.
为了评估脱氧黄苷在DNA中的碱基配对特性和诱变性，将该修饰碱基掺入合成的c-Ha-ras基因中并进行了DNA转染实验。含有脱氧黄苷的ras基因表现出非常高的集落形成活性。对转化体的基因分析表明几乎都是由G到A的转换。这些结果表明，dTMP优先掺入到与脱氧黄苷相对的位点，并且脱氧黄苷可在哺乳动物细胞中诱导由G到A的转换。
