Im A-Rang, Lee Byeonghun, Kang Dae-Jung, Chae Sungwook
1 Herbal Medicine Research Division, Korea Institute of Oriental Medicine , Daejeon, Korea.
2 Bioprocess Engineering Team, Research Laboratories, ILDONG Pharmaceutical Co., Ltd. , Hwaseong, Korea.
J Med Food. 2018 Oct;21(10):1016-1023. doi: 10.1089/jmf.2017.4100. Epub 2018 Sep 27.
Photoaging is generally the result of chronic exposure to the sun and ultraviolet (UV) radiation, which causes skin damage. In this study, we developed a UVB-induced hairless mouse model to determine whether Lactobacillus acidophilus IDCC 3302 tyndallizate (ACT3302) can enhance photodamaged skin repair. Mice (6 weeks old) were divided into six groups containing normal, UVB-treated vehicle, and UVB-treated ACT3302 (1 × 10, 1 × 10, 1 × 10, and 1 × 10 cells). Epidermal thickness was increased by UVB, but the thickening was lessened by ACT3302 as was the transepidermal water loss (TEWL). However, ACT3302 increased capacitance and decreased TEWL. Skin tissue staining to evaluate skin collagen increases in the number of skin collagen bundles in UVB-treated ACT3302 mice. UVB irradiation increased matrix metalloproteinase (MMP) and proinflammatory cytokine expression and activated mitogen-activated protein kinases in hairless mice; these changes were also attenuated by ACT3302. We conclude that ACT3302 effectively suppressed wrinkle formation induced by UVB irradiation through MMP downregulation. Therefore, ACT3302 potentially prevents skin photoaging and wrinkle formation.
光老化通常是长期暴露于阳光和紫外线（UV）辐射的结果，这会导致皮肤损伤。在本研究中，我们建立了一种紫外线B（UVB）诱导的无毛小鼠模型，以确定嗜酸乳杆菌IDCC 3302溶菌产物（ACT3302）是否能增强光损伤皮肤的修复。将6周龄小鼠分为六组，分别为正常组、UVB处理的赋形剂组和UVB处理的ACT3302组（1×10、1×10、1×10和1×10个细胞）。UVB可增加表皮厚度，但ACT3302可减轻这种增厚，经表皮水分流失（TEWL）也有所减轻。然而，ACT3302增加了皮肤电容并降低了TEWL。对皮肤组织进行染色以评估皮肤胶原蛋白，结果显示在UVB处理的ACT3302小鼠中皮肤胶原束数量增加。UVB照射可增加无毛小鼠体内基质金属蛋白酶（MMP）和促炎细胞因子的表达，并激活丝裂原活化蛋白激酶；这些变化也被ACT3302减弱。我们得出结论，ACT3302通过下调MMP有效抑制了UVB照射诱导的皱纹形成。因此，ACT3302可能预防皮肤光老化和皱纹形成。
