Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Talanta. 2019 Jan 1;191:504-508. doi: 10.1016/j.talanta.2018.09.005. Epub 2018 Sep 4.
In this work, a fast and low-cost voltammetric methodology for determination of carlina oxide in plant extracts was developed. The best results were obtained using a boron-doped diamond electrode (BDDE). The voltammetric measurements of carlina oxide were performed in a 0.1 mol/L solution of sulphuric acid. After 30 s of stirring the solution, differential pulse voltammograms (DPVs) were recorded from 0.5 to 1.8 V. The amplitude was 75 mV and the scan rate was 175 mV/s. Measurements were recorded in non-deaerated solutions. The background current was subtracted from each registered voltammogram; then they were cut from 0.5 to 1.5 V. The detection and quantification limits were 0.28 and 0.93 μg/L, respectively, and repeatability expressed as the relative standard deviation of 0.1 mg/L of carlina oxide was 1.9% (n = 5). The results were compared with those obtained using gas chromatography with a flame ionization detector and high performance liquid chromatography with a photodiode array detector.
本工作开发了一种快速、低成本的伏安法测定植物提取物中卡林氧化物的方法。使用掺硼金刚石电极（BDDE）可获得最佳结果。在 0.1 mol/L 硫酸溶液中进行卡林氧化物的伏安测量。搅拌溶液 30 秒后，从 0.5 到 1.8 V 记录差分脉冲伏安图（DPVs）。振幅为 75 mV，扫描速率为 175 mV/s。在非脱气溶液中进行测量。从每个注册的伏安图中减去背景电流；然后将其从 0.5 到 1.5 V 截断。检测限和定量限分别为 0.28 和 0.93 μg/L，以 0.1 mg/L 卡林氧化物的相对标准偏差表示的重复性为 1.9%（n=5）。将结果与使用火焰离子化检测器的气相色谱法和使用光电二极管阵列检测器的高效液相色谱法进行了比较。
