Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, E-23071 Jaén, Spain.
Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, E-23071 Jaén, Spain.
Talanta. 2019 Jan 1;191:89-93. doi: 10.1016/j.talanta.2018.08.049. Epub 2018 Aug 18.
Zearalenone (ZEA), a mycotoxin produced by several Fusarium molds, can be found in many cereals and related products. The toxicity of ZEA has been reported for both humans and animals. Therefore, many countries have adopted regulations in foods and feed materials to limit the exposure to this contaminant. In this paper, we propose a multicommutated flow-through optosensor to quantify ZEA in different cereal samples. ZEA was retained and pre-concentrated on C silica gel, and the use of the multicommutated flow manifold allowed the automated retention/desorption of ZEA on the solid microbeads by the use of appropriate carrier/eluting solutions, hence increasing the selectivity and sensitivity of the system. The native fluorescence of ZEA was recorded on the solid phase at λ/λ of 265/465 nm/nm. A QuEChERS procedure was used to carry out the extraction of ZEA from different cereal samples (feedstuff materials). Recovery studies were performed to assess the accuracy of the method, obtaining recovery yields between 93% and 107% in all the analyzed samples. LC-MS was employed as reference method. The quantitation limit of the proposed method was low enough to fulfill the maximum residue levels established by the Commission of the European Communities, thus demonstrating its potential use for the analysis of ZEA in feedstuffs.
玉米赤霉烯酮（ZEA）是由几种镰刀菌产生的一种真菌毒素，可存在于许多谷物及相关产品中。ZEA 对人类和动物均具有毒性。因此，许多国家已在食品和饲料材料中采用法规来限制对这种污染物的接触。在本文中，我们提出了一种多切换流通式光传感器，用于定量测定不同谷物样品中的 ZEA。ZEA 在 C 硅胶上被保留和预浓缩，多切换流通池的使用允许通过使用适当的载体/洗脱溶液自动将 ZEA 保留/解吸在固体微珠上，从而提高了系统的选择性和灵敏度。ZEA 的固有荧光在固相上在 λ/λ 为 265/465nm/nm 处被记录。采用 QuEChERS 法从不同谷物样品（饲料材料）中提取 ZEA。进行了回收率研究以评估方法的准确性，在所有分析的样品中均获得了 93%至 107%的回收率。采用 LC-MS 作为参考方法。所提出方法的定量限足够低，可满足欧洲共同体委员会制定的最大残留限量，从而证明其在饲料中 ZEA 分析中的潜在用途。
