Ben Taheur Fadia, Mansour Chalbia, Mechri Sondes, Skhiri Sihem Safta, Jaouadi Bassem, Mzoughi Ridha, Chaieb Kamel, Zouari Nacim
Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
Laboratory of Microbial Biotechnology, Enzymatic, and Biomolecules (LMBEB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia.
Toxicon X. 2022 Mar 29;14:100121. doi: 10.1016/j.toxcx.2022.100121. eCollection 2022 Jun.
Zearalenone (ZEA) is a toxic metabolite of the genus , which causes hepatotoxicity and induces oxidative stress. Kefir is an important probiotic dairy-product showing important antioxidant potential. In this study, the effect of Kefir supplementation to mitigate ZEA toxicity in rats was investigated. Animals were divided into four groups of five rats each, which received sterile milk (200 μL/day) during the first week. Then, they were switched to Kefir (200 μL/day), ZEA (40 mg/kg b. w./day) and Kefir + ZEA for the second week. Hematological and biochemical parameters, as well as liver histological analysis were determined. Kefir administration prevented the changes occurred in the count of all blood cells, and improved the antioxidant enzymes in the liver, such as catalase, glutathione peroxidase and superoxide dismutase activities that increased by 6, 4.5 and 1.3 folds, respectively, compared to ZEA group. Interestingly, the concurrent regimen Kefir + ZEA removed ZEA residues in the serum and liver. Furthermore, the Kefir + ZEA group showed a reduction in the levels of bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and hepatic malonaldehyde by ∼82, 54, 66, 50 and 36%, respectively, compared to the ZEA group. The histopathological analysis showed a normal liver histological architecture in Kefir + ZEA group, while degenerative changes were observed in ZEA group. These results suggest that Kefir as probiotic consortium may have a hepatoprotective effect against ZEA poisoning.
玉米赤霉烯酮（ZEA）是镰刀菌属的一种有毒代谢产物，可导致肝毒性并诱导氧化应激。开菲尔是一种重要的益生菌乳制品，具有重要的抗氧化潜力。在本研究中，研究了补充开菲尔对减轻大鼠ZEA毒性的作用。将动物分为四组，每组五只大鼠，在第一周给予无菌牛奶（200μL/天）。然后，在第二周将它们分别改为给予开菲尔（200μL/天）、ZEA（40mg/kg体重/天）和开菲尔+ZEA。测定了血液学和生化参数以及肝脏组织学分析。给予开菲尔可防止所有血细胞计数发生变化，并改善肝脏中的抗氧化酶，如过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的活性，与ZEA组相比，其活性分别提高了6倍、4.5倍和1.3倍。有趣的是，开菲尔+ZEA联合用药可清除血清和肝脏中的ZEA残留。此外，与ZEA组相比，开菲尔+ZEA组的胆红素、丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶和肝脏丙二醛水平分别降低了约82%、54%、66%、50%和36%。组织病理学分析显示，开菲尔+ZEA组肝脏组织学结构正常，而ZEA组观察到退行性变化。这些结果表明，作为益生菌组合的开菲尔可能对ZEA中毒具有肝脏保护作用。
