来自耐氟喹诺酮大肠杆菌菌株的DNA促旋酶的纯化及特性
Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli.
作者信息
Sato K, Inoue Y, Fujii T, Aoyama H, Inoue M, Mitsuhashi S
出版信息
Antimicrob Agents Chemother. 1986 Nov;30(5):777-80. doi: 10.1128/AAC.30.5.777.
Abstract
Subunit A and B proteins of DNA gyrase were separately purified from fluoroquinolone-resistant Escherichia coli GN14181 (MIC of ofloxacin, 100 micrograms/ml) and susceptible strain KL-16. The supercoiling activities of reconstituted Ar+Br (r, resistant) and Ar+Bs (s, susceptible) were 250-fold more resistant to new fluoroquinolones than those of As+Bs and As+Br.
摘要
从耐氟喹诺酮的大肠杆菌GN14181（氧氟沙星的最低抑菌浓度为100微克/毫升）和敏感菌株KL-16中分别纯化出DNA促旋酶的A亚基和B亚基蛋白。重组后的Ar+Br（r，耐药）和Ar+Bs（s，敏感）的超螺旋活性对新型氟喹诺酮的耐药性比As+Bs和As+Br高250倍。
Zotero 插件