Zhu Genglin, Yu Gang, Zhang Xianghui, Liu Jinliang, Zhang Yanhua, Rollins Jeffrey A, Li Jingtao, Pan Hongyu
College of Plant Sciences, Jilin University, Changchun, China.
College of Plant Sciences, Jilin University, Changchun, China
mSystems. 2019 Sep 10;4(5):e00397-19. doi: 10.1128/mSystems.00397-19.
GATA transcription factors (TFs) are common eukaryotic regulators, and glutathione-dependent formaldehyde dehydrogenases (GD-FDH) are ubiquitous enzymes with formaldehyde detoxification activity. In this study, the formaldehyde dehydrogenase Fdh1 (SsFdh1) was first characterized as an interacting partner of a GATA TF, SsNsd1, in Genetic analysis reveals that SsFdh1 functions in formaldehyde detoxification, nitrogen metabolism, sclerotium development, and pathogenicity. Both SsNsd1 and SsFdh1 harbor typical zinc finger motifs with conserved cysteine residues. SsNsd1 regulates SsFdh1 in two distinct manners. SsNsd1 directly binds to GATA-box DNA in the promoter region of ; SsNsd1 associates with SsFdh1 through disulfide bonds formed by conserved Cys residues. The SsNsd1-SsFdh1 interaction and nuclear translocation were found to prevent efficient binding of SsNsd1 to GATA-box DNA. Site-directed point mutation of these Cys residues influences the SsNsd1-SsFdh1 interaction and SsNsd1 DNA binding capacity. SsFdh1 is regulated by and functions jointly with the SsNsd1 factor, providing new insights into the complex transcriptional regulatory mechanisms of GATA factors. is a pathogenic fungus with sclerotium and infection cushion development, making one of the most challenging agricultural pathogens with no effective control method. We identified important sclerotium and compound appressorium formation determinants, SsNsd1 and SsFdh1, and investigated their regulatory mechanism at the molecular level. SsNsd1 and SsFdh1 are zinc finger motif-containing proteins and associate with each other in the nucleus. On other hand, SsNsd1, as a GATA transcription factor, directly binds to GATA-box DNA in the promoter region of The SsNsd1-SsFdh1 interaction and nuclear translocation were found to prevent efficient binding of SsNsd1 to GATA-box DNA. Our results provide insights into the role of the GATA transcription factor and its regulation of formaldehyde dehydrogenase in stress resistance, fungal sclerotium and compound appressorium development, and pathogenicity.
GATA转录因子（TFs）是常见的真核生物调节因子，而谷胱甘肽依赖性甲醛脱氢酶（GD-FDH）是具有甲醛解毒活性的普遍存在的酶。在本研究中，甲醛脱氢酶Fdh1（SsFdh1）首先被鉴定为GATA TF SsNsd1的相互作用伙伴。遗传分析表明，SsFdh1在甲醛解毒、氮代谢、菌核发育和致病性方面发挥作用。SsNsd1和SsFdh1都具有带有保守半胱氨酸残基的典型锌指基序。SsNsd1以两种不同方式调节SsFdh1。SsNsd1直接结合到[基因名称]启动子区域的GATA-box DNA上；SsNsd1通过保守半胱氨酸残基形成的二硫键与SsFdh1结合。发现SsNsd1-SsFdh1相互作用和核转位会阻止SsNsd1与GATA-box DNA的有效结合。这些半胱氨酸残基的定点突变会影响SsNsd1-SsFdh1相互作用和SsNsd1的DNA结合能力。SsFdh1受SsNsd1因子调控并与其共同发挥作用，为GATA因子复杂的转录调控机制提供了新见解。[病原菌名称]是一种具有菌核和侵染垫发育的致病真菌，是最难防治的农业病原菌之一，目前尚无有效的防治方法。我们鉴定了重要的菌核和复合附着胞形成决定因子SsNsd1和SsFdh1，并在分子水平上研究了它们的调控机制。SsNsd1和SsFdh1是含锌指基序的蛋白质，在细胞核中相互结合。另一方面，SsNsd1作为GATA转录因子，直接结合到[基因名称]启动子区域的GATA-box DNA上。发现SsNsd1-SsFdh1相互作用和核转位会阻止SsNsd1与GATA-box DNA的有效结合。我们的结果为GATA转录因子及其对甲醛脱氢酶在抗逆性、真菌菌核和复合附着胞发育以及致病性方面的调控作用提供了见解。
